FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_1_S9_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_1_S9_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18056494
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG215860.11954701726702868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT2750.0136.187321
CATGGCT2150.0133.994671
GCCCTTG550.0130.949341
TGCTGCT1850.0128.471921
CCTGCGG5000.0125.318521
GCCTGCT3600.0124.0381241
CTTGGCT2500.0120.997191
TCTGGCT3900.0108.956571
GGCCATT600.0108.03321
GACTGCT600.0108.03321
TGCAGCT203.9702145E-4108.03321
ACTGGCT2550.0107.327111
CTGCGGT9000.0106.432722
CTGTGCT4200.0106.31841
ACTGCGG4550.0106.054571
CCCTGCT14350.0104.8963551
ATGGCTC8400.0103.746182
CAGTGCT1750.0102.888761
CTGGCTC11600.0101.82442
GCTGGCT1350.0101.3644941
Produced by FastQC (version 0.11.5)