FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_1_S9_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_1_S9_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18056494
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCAT100.007210649144.196551
CATGGCT3200.0141.943471
CCAGGCT3000.0139.391
CCTGCGG8800.0136.822861
CTTGGCT3600.0136.185621
CCTGGCT4100.0131.887081
GCCCTGT550.0131.087771
CTGTGCT2100.0127.030291
GCTGGCT1750.0123.5970461
ACTGGCT4500.0123.3681641
TCTGGCT6400.0121.665831
GCCATGG301.13963324E-7120.163791
ACTGCGG7050.0117.607111
GCCTGCT3250.0117.5756451
CTTGCGG3450.0117.029091
CTGCGGA10950.0116.542422
GGCCACT501.8189894E-12115.357241
GCCCTGG256.649032E-6115.357241
GCCAGCT1500.0115.357241
CTAGGCT2950.0114.868441
Produced by FastQC (version 0.11.5)