FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_1_S9_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_1_S9_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18172174
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG241890.13311010559330985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCT4650.0131.652131
CTTGGCT2500.0129.638631
CCTGGCT2450.0129.344651
CATGGCT1700.0127.0966951
ACTGGCT2750.0125.710191
CTGTGCT5250.0122.093521
CCTGCGG4450.0118.147561
TGCTGCT1850.0116.791561
TGCGGCT700.0113.1765751
CCAGGCT1850.0112.8985061
CTGCGGT9200.0112.7286152
GTCAGCT458.913048E-11112.033381
ACTGCGG3750.0111.393191
TGCCTTG203.9703635E-4108.032191
TGCAGCT405.0476956E-9108.032191
CTGGCTC10350.0107.1617662
GCTGGCT1350.0106.698461
TTGGCTC13300.0106.677822
TGTGCTC16850.0106.001332
CTGGGCT553.6379788E-12104.758491
Produced by FastQC (version 0.11.5)