FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_1_S9_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_1_S9_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18172174
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGCT450.0144.214451
TGCCATG100.0072079753144.214431
CATGGCT3400.0139.972841
GCTGGCT2450.0138.328141
CTTGGCT5000.0135.561571
CCTGCGG9900.0135.474151
GGCCTAT800.0135.201031
CCTGGCT5800.0134.268621
ACTGGCT4150.0132.051771
GCCTAGG403.45608E-11126.187641
GCCTGCT2600.0124.800961
CTAGGCT3800.0123.3412861
TGCTCGG1000.0122.582261
CTGTGCT1600.0121.680931
ACTGCGG8250.0121.4897461
CCTGCGA3650.0120.507951
TCTGGCT5700.0118.913661
CCAGGCT3000.0117.7751161
GCCTCGG2500.0115.371551
CTGCGGT16400.0115.195682
Produced by FastQC (version 0.11.5)