FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_4_S4_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_4_S4_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15591852
Sequences flagged as poor quality0
Sequence length150
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT1800.0136.040241
CTTGGCT1600.0126.037281
GCCTGCT2500.0123.876641
CCTGCGG1700.0122.859861
CTGTGCT2150.0120.59381
CATGGCT1150.0118.9917141
GGCCCTA458.913048E-11112.0331341
ACTGGCT2250.0112.0331341
TGCTGCT1050.0109.746741
TGCCCGT203.9703876E-4108.031951
GCCCTGT405.0476956E-9108.031951
GCAGGCT405.0476956E-9108.031951
TCTGGCT2550.0107.325861
GGCTGCT850.0101.677131
CTGGCTC8400.0101.1727752
TAGGCTC5700.0101.082522
TGTGCTC9050.0100.272752
GCTGGCT800.099.029291
TTGGCTC9550.098.793622
ACTGCGG1950.096.02841
Produced by FastQC (version 0.11.5)