FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_4_S4_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_4_S4_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15591852
Sequences flagged as poor quality0
Sequence length150
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGCT1200.0132.186391
CTTGGCT2150.0130.789061
CTGTGCT1550.0130.248171
CCTGCGG3250.0128.673741
CCTGGCT3650.0128.400221
CAGTGCT450.0128.180741
CATGGCT1550.0125.596441
TAGGCTC6750.0121.7717062
GGCCCTA550.0117.984551
GCCTGCT2350.0116.589921
ACTGGCT2300.0115.9896241
TGTGCTC5300.0111.553512
CTAGGCT2200.0111.429851
CTGCGGT6600.0111.429842
CCAGGCT2400.0111.156731
TTGGCTC12650.0110.004912
CTGGCTC11150.0108.637492
ACTGCGG3950.0107.696161
TCTGGCT3100.0104.6637041
TGGCTCT36450.0104.287483
Produced by FastQC (version 0.11.5)