FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_4_S4_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_4_S4_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15706000
Sequences flagged as poor quality0
Sequence length150
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161590.10288424805806698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGCT1150.0131.527151
CCTGGCT2150.0120.602951
CATGGCT850.0118.6323241
GCCTGCT2650.0116.873621
GCTGGCT1250.0115.242821
CCTGCGG1700.0114.3954541
TTGGCTC10100.0109.1098562
CTGTGCT1850.0109.013481
GGCCATC203.969191E-4108.040141
ACTGGCT1950.0107.116721
CTGGCTC8600.0106.3651052
GCCAGCT900.0104.0386661
TGCAGAC5200.0103.84873
CCAGGCT1250.0103.7185441
GCAGGCT352.8736213E-7102.895381
TGGCTCT29200.0100.605223
TGTGCTC9500.0100.0792852
GCTTGCT650.099.729361
CTGCGGT4500.097.636282
TAGGCTC4050.096.035692
Produced by FastQC (version 0.11.5)