FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_4_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_4_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15706000
Sequences flagged as poor quality0
Sequence length150
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTACC100.0072102104144.199421
TCTGGCT3400.0137.837681
ACTGGCT2350.0134.99521
CTTGGCT2700.0133.517971
CCTGGCT2700.0133.517971
GCTGGCT1600.0130.680731
CCTGCGG3350.0122.677111
GCCTGCT2000.0122.56951
CATGGCT1550.0120.941441
CTAGGCT1550.0120.941441
GGCCATT301.1394877E-7120.166181
GGCCCTA256.6483462E-6115.359531
ACAGGCT750.0115.359531
GGCCTAA1200.0114.157871
CTGGCTC12000.0112.956212
GCAGGCT800.0108.149571
GGCCTAC203.9532405E-4108.149571
TTGGCTC14050.0107.251522
CATGCGG1150.0106.582181
TAGGCTC5550.0106.5256962
Produced by FastQC (version 0.11.5)