FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_3_S3_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_3_S3_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13670957
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG263560.19278825908091146No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCTG100.0072309724144.060731
CCTGGCT750.0134.456681
CCTGCGG1150.0125.27021
CTTGGCT501.8189894E-12115.248591
GCCCTTG256.680195E-6115.248591
GGCCACT203.9683902E-4108.045551
CTGGCTC3400.0108.045552
GCCCTGT750.0105.644541
GGCCATT352.8727482E-7102.900521
GGCCAAC352.8727482E-7102.900521
GCCAGCT502.055458E-10100.8425141
TGGCTCT11250.096.637273
GGCCCTT301.6526674E-596.040481
TGCAGAC2100.095.9972843
TTGGCTC3450.093.952652
CTGCGGT3350.090.3067252
TGCTGCT406.363098E-790.037961
ACTGCGG1850.089.551271
ACTGGCT651.6370905E-1188.6527561
TCTGGCT1550.088.295291
Produced by FastQC (version 0.11.5)