FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_3_S3_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_3_S3_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13670957
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGG100.0072097285144.202581
GCTGGCT550.0144.202581
CCTGGCT1750.0131.842351
TCTGGCT1850.0128.613111
GCCAGCT450.0128.180071
CCTGCGG2400.0123.1730351
GCCCTGT950.0121.433751
GGCCTAA600.0120.1688161
CTTGGCT1500.0115.362071
GGCCGAA256.647593E-6115.362061
ACTGGCT1650.0113.614151
CTGGCTC6650.0110.5914462
GGCTAAC405.007678E-9108.151931
TGTGCTC1800.0108.1519242
TAGGCTC2550.0107.445052
CCAGCGG1950.0107.227551
GCTGCGA950.0106.2545241
TTGGCTC7050.0105.339472
GGCCTTT553.6379788E-12104.87461
ACAGGCT700.0103.001841
Produced by FastQC (version 0.11.5)