FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_3_S3_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_3_S3_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13688650
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG292280.21351995996683384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGCT450.0144.048511
GGCTGCT100.0072328085144.04851
GTGCCAC100.0072328085144.04851
GGCCAAT301.14661816E-7120.040411
CCTGGCT700.0113.180961
GACTGCT203.969731E-4108.036381
CCTGCGG1700.0105.9180151
ACTGGCT553.6379788E-12104.762541
GCTGGCT700.0102.891781
CTGCGGT4450.0101.9665452
CCAGGCT502.055458E-10100.833951
TTGGCTC3450.0100.207282
GCTGCGA800.099.033341
CTGCGAT2350.098.075222
CTTGGCT451.1437805E-896.032331
GGCCTTT451.1437805E-896.032331
CTAGGCT451.1437805E-896.032331
ACTGCGG1900.094.768751
GCCCTGT554.3837645E-1091.667231
TCTGGCT1100.091.667231
Produced by FastQC (version 0.11.5)