FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_3_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_3_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13688650
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT1600.0126.193861
CCTGCGG2750.0125.86611
TCTGGCT1800.0124.190791
GCTGGCT700.0123.6184841
GCCCTTG351.9772415E-9123.6184841
CATGGCT850.0118.77071
TTGGCTC6150.0116.080772
ACAGGCT458.913048E-11112.1723251
TAGGCTC2550.0110.287072
CTTGGCT1250.0109.608381
CTGCGGT5850.0108.474342
GCCGGAC203.950816E-4108.166171
GGCCCCT203.950816E-4108.166171
GGCCATT203.950816E-4108.166171
GGACCAA203.950816E-4108.166171
CTAGGCT600.0108.166171
CTGTGCT203.950816E-4108.166171
ACTGGCT1150.0106.598551
GGCCAAT750.0105.762481
GGCCTAA553.6379788E-12104.888411
Produced by FastQC (version 0.11.5)