FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_2_S2_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_2_S2_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17682252
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG248720.14066081628064117No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCATC100.0072312504144.058991
GGCCAAT950.0121.3128361
GCTGGCT501.8189894E-12115.247191
CCTGCGG1950.0114.508431
GCCCTGT1150.0106.478381
CTGCGGT4850.0105.4452442
ATGGCTC3650.0104.590772
GCCTGCT850.0101.68871
CTGGCTC4250.0101.68872
CCTGGCT1000.0100.841291
GCACCTG301.6527796E-596.039331
TCTGCGG4200.094.324331
ACTGCGG2150.093.805851
TGTGCTC5000.093.638342
TGGCTCT14650.091.901533
TGCCGAA1550.088.294221
TTGGCTC4500.088.036052
GGCCTTA502.375782E-886.4353941
GGCCTCC259.6353644E-486.4353941
CTGCGGA3550.085.2179952
Produced by FastQC (version 0.11.5)