FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_2_S2_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_2_S2_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17682252
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAC100.0072106067144.196821
GGCCATC306.7666406E-10144.196821
CCTGGCT2100.0144.196821
ACTGGCT1150.0131.657961
GCCCTGT1350.0128.174961
GCTGCGA1300.0127.558731
CTTGGCT1450.0124.307611
GCTGGCT700.0123.5972751
CCTGCGG3100.0120.939271
TCTGGCT2550.0118.750331
CATGGCT1250.0115.357461
GGCCCTA750.0115.357461
CTGGCTC7400.0113.993432
GGCCATA950.0113.839591
GGCCAAT458.913048E-11112.153081
CTAGGCT900.0112.153081
GGCCTAA1800.0112.153081
TAGGCTC3450.0108.670072
CTGCGGT6300.0107.57542
TTGGCTC7950.0107.0142
Produced by FastQC (version 0.11.5)