FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_2_S2_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_2_S2_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17734461
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG277740.15661034186491488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA650.0121.880771
CCTGGCT1050.0109.745451
CTTGGCT553.6379788E-12104.757031
GCCCTTG553.6379788E-12104.757031
GCTGCGA1250.0103.709461
GCTGGCT700.0102.886361
CCTGCGG1850.0101.2179261
CTGGCTC4200.0101.171592
CATGGCT650.099.720631
ATGGCTC2600.099.720632
GCTGCGG2500.097.947821
TGCAGAC2000.097.20133
TGCTGCT900.096.0272751
ACTGGCT750.096.027271
TGTGCTC4250.094.897532
GCCTGCT1150.093.939721
CTGCGGT4900.092.5977252
ACTGCGG2650.092.40361
TGGCTCT14050.092.243223
TCTGGCT1800.092.026141
Produced by FastQC (version 0.11.5)