FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_2_S2_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_2_S2_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17734461
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCATC100.0072093895144.204971
CCTGGCT1400.0139.054781
GGCCATA900.0136.193571
ACTGGCT1700.0135.722321
CTTGGCT1650.0131.095411
GCCCTGT1000.0129.784471
CATGGCT1100.0124.540641
CCTGCGG2000.0122.574221
GCTGGCT1000.0115.363971
GGCACTA256.6471002E-6115.363971
GGCCGAA256.6471002E-6115.363971
GCAGCGG1550.0111.642551
ACTGCGG3250.0110.9268951
ATGGCTC5600.0110.72882
GCCTGTT850.0110.274381
TTGGCTC8400.0109.870452
TCTGGCT2100.0109.870451
CTTGCGG1650.0109.2461851
GGGCTCA203.9526442E-4108.1537251
GGCCTAT600.0108.153721
Produced by FastQC (version 0.11.5)