FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_1_S1_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_1_S1_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19571235
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG325890.16651478560244154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT1550.0125.4664151
GCCTGCT2600.0121.8918841
CATGGCT1500.0120.045041
ATGGCTC5650.0116.008122
ACTGGCT2600.0113.581081
GCTGGCT1350.0112.042041
GGCCCAT650.0110.810811
CTGGCTC8450.0110.810812
GGCTGCT800.0108.0405351
CCTGCGG3700.0105.1205141
CTTGGCT1650.0104.766571
ACTGCGG3600.0104.039031
TGGCTCT27650.0103.37423
CTAGGCT1200.0102.0382841
TGTGCTC10750.0100.502822
TCTGGCT3000.098.436931
CCCTGCT7500.097.956751
CCAGGCT1200.096.036031
CCAGCGG2400.096.036031
GGCCACT451.1435986E-896.0360261
Produced by FastQC (version 0.11.5)