FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_1_S1_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_1_S1_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19571235
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCAA202.1910255E-6144.201221
CCTGGCT4050.0138.860431
CTAGGCT2600.0135.881911
CCTGCGG7550.0131.786541
CATGGCT2200.0127.814711
CAGTGCT750.0124.974381
ACTGGCT3400.0122.995141
TCTGGCT4000.0117.163481
CCTGCGA3550.0113.736171
TAGGCTC7950.0112.458812
CTTGGCT2950.0112.428061
GCTGGCT1950.0110.924011
CTGGCTC15150.0109.935582
ACTGCGG7450.0109.360651
CTGCGGA11250.0108.952032
CCAGGCT2400.0108.150911
TTGGCTC19150.0107.303782
ATGGCTC10650.0106.2891542
CATGCGG2450.0105.943741
GCCATGG553.6379788E-12104.8736041
Produced by FastQC (version 0.11.5)