FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_1_S1_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_1_S1_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19624894
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG361460.18418443432102105No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT1800.0132.03071
GCCTGCT2900.0119.200121
CTTGGCT2000.0111.6259461
ACTGCGG4200.0111.454481
ACTGGCT1500.0110.4256741
TTGGCTC10000.0110.1856162
CCTGCGA2100.0109.73981
CCTGCGG3500.0109.0539251
GCTTGCT405.0495146E-9108.025121
CAGTGCT950.0106.129941
CTGGCTC7850.0105.502232
CTGTGCT2200.0104.751621
CTGCGGT7850.0104.5848162
CTAGGCT900.0104.0241851
TGGCTCT26450.0103.714053
CCCTGCT7350.0102.881061
CTGCGAT5150.0102.0819852
TGTGCTC9950.099.158732
GCCCTGT950.098.549231
CATGGCT1100.098.204641
Produced by FastQC (version 0.11.5)