FastQCFastQC Report
Mon 22 Jul 2019
16C_32psu_1_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_1_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19624894
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGCT151.257532E-4144.206791
ACTGGCT2800.0139.056531
CCTGCGG8000.0132.489991
CATGGCT2200.0131.097081
CTTGGCT3750.0130.747481
CCTGGCT4350.0129.288831
CCAGGCT2850.0129.027131
GGCCTTA403.45608E-11126.180931
GCTGGCT1950.0122.021121
ACTGCGG6800.0119.818881
CCTGCGA4650.0119.397021
CTGTGCT1100.0117.987371
CATGCGG2400.0114.1637041
TCTGGCT3800.0113.847461
CTGCGGA9700.0113.73012
CTGCGGT14600.0113.587532
ATGGCTC10050.0111.921682
CCAGCGG6000.0111.760261
GCCCTTG650.0110.92831
TTGGCTC17650.0110.299812
Produced by FastQC (version 0.11.5)