FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_4_S16_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_4_S16_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17208353
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG333440.19376636450914275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGCT1150.0125.258481
GCCTGCT1900.0125.0936661
CCAGGCT700.0123.469071
CCTGCGG1650.0122.221911
CCTGGCT1750.0119.353431
CTGTGCT2100.0116.609681
GCCCTTG405.0458766E-9108.035431
ACTGGCT1350.0106.7016751
CTGGCTC6200.0104.550422
GCTGGCT1150.0100.206781
TTGGCTC7750.097.580392
TGCAGAC5250.097.368863
TCTGGCT1850.097.329221
CAGTGCT750.096.03151
TGGCTCT22550.095.78463
CCCTGCT7600.094.767931
CATGGCT700.092.60181
GCAGGCT700.092.60181
TGTGCTC8250.092.5394442
CTAGGCT1100.091.6664351
Produced by FastQC (version 0.11.5)