FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_4_S16_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_4_S16_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17208353
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGCT351.2732926E-11144.205151
CCTGGCT4300.0132.467511
CCTGCGG4650.0131.80041
CTTGGCT3000.0129.784641
ACTGGCT2800.0128.75461
GCTGGCT1550.0120.946261
CATGGCT1750.0119.4842761
CCAGGCT2300.0119.125991
CTAGGCT2750.0117.986031
TCTGGCT4200.0116.737511
CTGCGGA6250.0111.9032062
TTGGCTC15800.0111.804632
TAGGCTC8200.0109.0331652
GGCAGCT203.952622E-4108.153861
GCCATGT203.952622E-4108.153861
GGCCCTA405.007678E-9108.153861
GCCTCGG1550.0106.990921
CTGGCTC14900.0106.9440842
ACAGGCT1500.0105.750451
CCTGCGA2250.0105.750441
Produced by FastQC (version 0.11.5)