FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_4_S16_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_4_S16_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17274454
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG370570.21451908118195806No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCT100.0072334534144.044311
GCCCTGC100.0072334534144.044311
GCTGGCT800.0135.041531
CCTGGCT1600.0130.540151
GGCTGCT500.0129.639881
CATGGCT1350.0117.369431
CTTGGCT2000.0115.235441
GCCTGCT1700.0114.3881151
ACTGGCT1650.0113.489451
TGCTGCT1050.0109.748031
TGTGCTC7500.0109.473672
CCTGCGG3100.0109.194881
CCAGGCT1400.0108.033231
GGCAGCT203.970208E-4108.0332261
GGCAACT203.970208E-4108.0332261
TGCCTTG203.970208E-4108.0332261
GCATGCT203.970208E-4108.0332261
GGCTACC203.970208E-4108.0332261
CTGTGCT2450.0105.828471
TAGGCTC3700.0103.166872
Produced by FastQC (version 0.11.5)