FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_4_S16_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_4_S16_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17274454
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTTG306.7666406E-10144.21281
CAGGCCT100.007208217144.21282
CTGGCTT100.007208217144.21282
CATGGCT2450.0138.326571
CCTGGCT5000.0137.002151
CTTGGCT3200.0135.199511
CCTGCGG5050.0131.362151
ACTGGCT3100.0125.60471
CTAGGCT2450.0123.610981
CTGGGCT700.0123.610971
GGCCATA600.0120.177341
GCTGGCT1700.0118.763481
ACAGGCT1100.0117.9922941
CTGTGCT1350.0117.506721
TCTGGCT4200.0116.74371
CCAGGCT2100.0113.310061
CTGCGGT8100.0113.055722
CATGCGG1300.0110.932921
CTGGCTC15850.0110.093052
TTGGCTC14950.0109.485982
Produced by FastQC (version 0.11.5)