FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_3_S15_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_3_S15_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14622552
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG398820.2727430888944693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGCT750.0124.840551
CCTGGCT1600.0117.038011
TGCTGCT1050.0116.60931
CTTGGCT1000.0108.0350951
ACTGGCT1100.0104.76131
CTGTGCT1800.0104.033791
TGTGCTC6050.0102.3803562
TAGGCTC4450.0100.34722
GGCCTAA650.099.724691
TTGGCTC5900.098.879572
GCCTGCT1400.097.746031
CCAGGCT900.096.031191
TGGCTCT19250.093.128643
GGCTGCT554.3837645E-1091.666141
TGCAGAC5000.090.716263
CCTGCGG1350.090.696121
ACTGCGG1050.089.1718141
CTGCGGT2100.089.1718142
ATGGCTC4300.088.773022
CATGCGG651.6370905E-1188.644171
Produced by FastQC (version 0.11.5)