FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_3_S15_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_3_S15_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14622552
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT3050.0139.473821
CTTGGCT2200.0134.36981
CATGGCT2400.0129.180731
ACTGGCT2250.0124.974831
TCTGGCT4400.0122.89921
CCAGGCT1750.0119.481441
CCTGCGG3050.0118.198141
GCCTTGG256.6478005E-6115.361391
CTAGGCT1700.0114.513141
CTGTGCT950.0113.843471
GCTGGCT1350.0112.1569061
CTGGCTC12550.0110.880222
TTGGCTC11600.0109.3944242
CTGGGCT405.009497E-9108.15131
TAGGCTC5700.0106.253912
CCAGCGG1900.0106.25391
TGGCTCT38750.0104.4128953
ATGGCTC9250.0103.669352
CAGGCTC4250.0103.485952
CATGCGG700.0103.0012361
Produced by FastQC (version 0.11.5)