FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_3_S15_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_3_S15_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14705097
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG434450.2954417777727002No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTACC100.007232619144.049791
GCCTGCT1850.0128.476851
CCTGCGG1250.0121.001841
CATGGCT1050.0116.611741
CTTGGCT1300.0116.3479161
CCTGGCT1750.0115.2398451
ACTGGCT1200.0108.0373461
TTGGCTC6000.0103.235692
CAGTGCT700.0102.892711
ATGGCTC5050.0101.2627262
TGTGCTC6100.098.00112
CTGCGGT2900.096.861072
GCCCTGT451.1437805E-896.03321
GGCCTAA900.096.03321
TGGCTCT20200.095.884673
TAGGCTC4250.093.2086942
TGCAGAC4100.093.0765463
CCTTGCT3300.091.668051
TGCTGCT1200.090.031131
CTGGCTC7150.089.653372
Produced by FastQC (version 0.11.5)