FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_3_S15_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_3_S15_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14705097
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACTA100.007207073144.220382
GGCATGC100.007242427143.984517
CCTGGCT3200.0137.460051
CTTGGCT2550.0132.908981
GCTGGCT1100.0131.109441
CATGGCT2050.0126.632521
CTAGGCT2000.0122.587321
TCTGGCT3750.0121.145121
GGCCAAT301.1385055E-7120.1836551
ACTGGCT2100.0120.183651
GGCCCTA501.8189894E-12115.3763051
TAGGCTC6450.0111.7987442
ACAGGCT1100.0111.443021
CTGTGCT1300.0110.938751
TTGGCTC12650.0110.587962
CCTGCGG2300.0109.7328951
CTGGCTC11750.0109.2392652
GCCTTGG405.00404E-9108.165291
GCCATGT203.95095E-4108.165291
ATGGCTC8950.0107.158162
Produced by FastQC (version 0.11.5)