FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_2_S14_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_2_S14_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17174050
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205780.11982031029372803No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCGG2000.0118.846991
GGCCCCT203.9688204E-4108.0427251
GCTGGCT1400.0102.897831
TGCAGAC4750.0101.556073
CTTGGCT1900.098.5652851
TGTGCTC7650.096.037972
GCAGGCT451.1434167E-896.037971
CTGGCTC6250.095.653822
ACTGGCT1750.094.6661
TTGGCTC7400.092.4689942
CTGGGCT950.090.983341
CTGTGCT1900.090.983341
GCAGCGG2150.090.454371
GCCCTTG406.364153E-790.03561
CAGTGCT800.090.03561
CTGCGGT3850.089.801742
GCCTGCT1650.087.307251
GCCCTGG259.6359017E-486.434181
TCTGGCT2500.086.434171
TGGCTCT22050.085.5494843
Produced by FastQC (version 0.11.5)