FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_2_S14_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_2_S14_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17174050
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATGG400.0144.207571
CCTGGCT3150.0135.051511
CTTGGCT2100.0130.47351
ACTGGCT1950.0129.417041
GCTGGCT1450.0124.316861
GCTGCGA950.0121.437951
CCTGCGG4050.0121.063131
GGCCTTT600.0120.1729661
TCTGGCT3350.0116.226991
TGTGCTC4350.0112.713952
CATGGCT1600.0112.6621551
CTAGGCT1600.0112.6621551
GGCCACT405.007678E-9108.155671
GCCCTTG600.0108.155671
CTGTGCT1400.0108.155661
TTGGCTC11350.0107.996852
CTGGCTC11500.0106.5881962
CTGCGGT6700.0106.5414052
GGCCTAT553.6379788E-12104.878221
CTGCGGA5050.0104.229222
Produced by FastQC (version 0.11.5)