FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_2_S14_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_2_S14_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17243447
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG224690.13030457309376717No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGG151.2629564E-4144.050961
CCTGGCT1300.0132.970111
CATGGCT700.0123.4722441
TGCCTTG256.682496E-6115.240771
GGCCAAT458.913048E-11112.0396351
CCTGCGG2000.0111.6394961
CTTGGCT1050.0109.753111
GCCTGCT1850.0109.0115361
ACTGGCT1200.0108.038231
CAGTGCT750.0105.6373751
ATGGCTC5150.0102.094372
TGTGCTC8450.099.727592
CTGTGCT1900.098.561191
TTGGCTC6750.098.168062
TGGCTCT21650.098.109943
GCCAGCT607.2759576E-1296.033981
TCAGGCT900.096.033971
TGCAGAC3750.096.003623
CTGCGGT4150.095.455452
CTGGCTC6300.094.890712
Produced by FastQC (version 0.11.5)