FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_2_S14_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_2_S14_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17243447
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGCT2400.0132.185351
CCTGGCT3150.0130.468661
CCTGCGG3900.0129.412221
GCTGGCT1200.0126.176921
TCTGGCT4350.0124.3122251
ACTGGCT2100.0123.6018751
CATGGCT1650.0122.353361
ACTGCGG3550.0119.829981
GGCCAAT550.0117.9836041
CTAGGCT1550.0111.64041
CTGGCTC12250.0110.064532
TTGGCTC12400.0108.151642
TAGGCTC5650.0107.194542
CTGCGGT7100.0105.612862
GGCCTAA1100.0104.874311
TGGCTCT39000.0104.303313
GGCCCTA700.0103.001561
ATGGCTC8700.0101.107282
CTTGCGG2250.099.339281
CTGCGGA4750.098.664662
Produced by FastQC (version 0.11.5)