FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_1_S13_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_1_S13_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22055675
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG308790.14000478334941008No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATCC100.0072321338144.053191
CCTGGCT1700.0135.579481
CCTGCGG2750.0120.480861
GGCCACT850.0118.632041
GCTGGCT1400.0118.3294141
CTTGGCT1550.0116.1719361
GGCCTTA950.0106.144461
GGCCAAT1300.0105.269641
CTGGCTC8200.0104.526412
TGTGCTC8850.0101.732482
TTGGCTC9700.0100.985742
GCCTGCT2000.0100.8372341
TGGCTCT28050.099.076373
CCAGGCT1100.098.218091
ACTGGCT2000.097.23591
CTGTGCT2600.096.958881
TAGGCTC5400.093.367812
CATGGCT1100.091.670211
GCCAGCT554.3837645E-1091.670211
TGCAGAC5300.091.0153353
Produced by FastQC (version 0.11.5)