FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_1_S13_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_1_S13_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22055675
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGCT3300.0133.281831
CCTGCGG6750.0131.388211
CCTGGCT3250.0128.676641
CATGGCT2100.0127.039121
CTTGGCT3300.0126.726981
TTGGCTC18050.0117.0430152
GCCTGCT1850.0116.924241
GCTGGCT1450.0114.3707351
GGCCATA1200.0108.154941
GCCATGT203.9524815E-4108.154931
CTGGCTC14650.0107.293632
CTGGGCT950.0106.2574841
TGGCTCT51150.0105.843983
CCAGCGG3500.0103.004691
CTTGCGG2450.0103.004691
ATGGCTC11450.0102.644872
CATGCGG2150.0100.609241
CCAGGCT2150.0100.609241
TCTGGCT5450.0100.54771
GGCCCAA1150.0100.317621
Produced by FastQC (version 0.11.5)