FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_1_S13_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_1_S13_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22159935
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG341760.15422427908746122No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGCT2100.0123.463031
GCCCTTG351.9936124E-9123.463031
CCTGGCT1600.0121.533921
GCCTGCT2400.0120.03351
GGCCATA1500.0120.033491
CTGTGCT2500.0115.2321551
TCTGGCT2450.0108.765041
GGCCCAT203.9706734E-4108.030151
TGTGCTC12300.0107.15162
CATGGCT950.0106.134881
ACTGGCT1700.0105.911911
CCTGCGG3300.0104.756511
GCCCTGT700.0102.885861
TTGGCTC10150.098.628282
ACTGCGG2900.096.8546141
GCTGGCT607.2759576E-1296.02681
CCAGGCT1050.096.02681
CAGTGCT607.2759576E-1296.02681
CTGGCTC7150.095.690822
TGGCTCT28600.094.15423
Produced by FastQC (version 0.11.5)