FastQCFastQC Report
Mon 22 Jul 2019
16C_26psu_1_S13_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_1_S13_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22159935
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGCT2400.0135.202261
CTTGGCT3650.0134.337941
CATGGCT2050.0133.663361
CCTGCGG5750.0132.929291
CCTGGCT4400.0132.744031
GCTGGCT1550.0130.259371
TCTGGCT4150.0121.627731
CTAGGCT1850.0120.829391
GCCTGCT1200.0120.179781
GGCCCAT256.6446682E-6115.37261
ATGGCTC11400.0111.3244252
GCCATGG405.005859E-9108.1618041
ACAGGCT1400.0108.16181
CTGGCTC15150.0106.614942
GGCCCTA950.0106.264231
TAGGCTC8350.0106.218772
TGGCTCT51800.0105.356683
CAGTGCT553.6379788E-12104.884171
TTGGCTC18700.0104.112972
CAGGCTC5900.0103.8842242
Produced by FastQC (version 0.11.5)