Bismark report for: /gscratch/scrubbed/strigg/analyses/20200930/Tank2-025-026_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20200930/Tank2-025-026_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v01/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 65947406 Number of paired-end alignments with a unique best hit: 31748771 Mapping efficiency: 48.1% Sequence pairs with no alignments under any condition: 30922473 Sequence pairs did not map uniquely: 3276162 Sequence pairs which were discarded because genomic sequence could not be extracted: 7 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 15798071 ((converted) top strand) GA/CT/CT: 87134 (complementary to (converted) top strand) GA/CT/GA: 85413 (complementary to (converted) bottom strand) CT/GA/GA: 15778146 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1296491035 Total methylated C's in CpG context: 29427344 Total methylated C's in CHG context: 2816200 Total methylated C's in CHH context: 18823480 Total methylated C's in Unknown context: 170971 Total unmethylated C's in CpG context: 121587680 Total unmethylated C's in CHG context: 223639272 Total unmethylated C's in CHH context: 900197059 Total unmethylated C's in Unknown context: 2947605 C methylated in CpG context: 19.5% C methylated in CHG context: 1.2% C methylated in CHH context: 2.0% C methylated in unknown context (CN or CHN): 5.5% Bismark completed in 0d 4h 36m 40s