FastQCFastQC Report
Fri 20 Mar 2020
EPI-113_S30_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-113_S30_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25934052
Sequences flagged as poor quality0
Sequence length20-85
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAATATAAAAA838400.3232815296275337No Hit
AACCAAACTCAAAAACTAAATATCACTAAACAAACCAAAAAATATAAAAA330300.12736150910779387No Hit
ACACCCAATTTTTAAACCTAACTCATATAAAAATCAATCATCAAATATCC280550.10817823608898448No Hit
CAACAACACACACCAAACAACAATCTATAAACTATTTAAAACAATTAAAA260070.10028128269350274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)