Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/20bp_clip/EPI-167_S10_L002_R1_001_val_1.20bp-trim.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/20bp_clip/EPI-167_S10_L002_R2_001_val_2.20bp-trim.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/OFS/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 24477306 Number of paired-end alignments with a unique best hit: 10276418 Mapping efficiency: 42.0% Sequence pairs with no alignments under any condition: 11771224 Sequence pairs did not map uniquely: 2429664 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5086774 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 5189644 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 256660837 Total methylated C's in CpG context: 8579400 Total methylated C's in CHG context: 1100210 Total methylated C's in CHH context: 4713706 Total methylated C's in Unknown context: 34055 Total unmethylated C's in CpG context: 24327012 Total unmethylated C's in CHG context: 52134228 Total unmethylated C's in CHH context: 165806281 Total unmethylated C's in Unknown context: 540239 C methylated in CpG context: 26.1% C methylated in CHG context: 2.1% C methylated in CHH context: 2.8% C methylated in unknown context (CN or CHN): 5.9% Bismark completed in 0d 0h 43m 57s