Bowtie seems to be working fine (tested command '/gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/bowtie2 --version' [2.3.4]) Output format is BAM (default) Alignments will be written out in BAM format. Samtools path provided as: '/gscratch/srlab/programs/samtools-1.9/samtools' Reference genome folder provided is /gscratch/srlab/strigg/data/Pgenr/OFS/ (absolute path is '/gscratch/srlab/strigg/data/Pgenr/OFS/)' FastQ format assumed (by default) Each Bowtie 2 instance is going to be run with 4 threads. Please monitor performance closely and tune down if needed! Input files to be analysed (in current folder '/gscratch/scrubbed/strigg/analyses/20200316/TG_EPI-Test2'): EPI-167_S10_L002_R1_001_val_1.fq.gz EPI-167_S10_L002_R2_001_val_2.fq.gz Library is assumed to be strand-specific (directional), alignments to strands complementary to the original top or bottom strands will be ignored (i.e. not performed!) Output will be written into the directory: /gscratch/scrubbed/strigg/analyses/20200316/TG_EPI-Test2/ Setting parallelization to single-threaded (default) Current working directory is: /gscratch/scrubbed/strigg/analyses/20200316/TG_EPI-Test2 Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/strigg/data/Pgenr/OFS/ chr Scaffold_01 (89643857 bp) chr Scaffold_02 (69596280 bp) chr Scaffold_03 (57743597 bp) chr Scaffold_04 (65288255 bp) chr Scaffold_05 (67248332 bp) chr Scaffold_06 (61759565 bp) chr Scaffold_07 (43120122 bp) chr Scaffold_08 (61151155 bp) chr Scaffold_09 (38581958 bp) chr Scaffold_10 (53961475 bp) chr Scaffold_11 (51449921 bp) chr Scaffold_12 (50438331 bp) chr Scaffold_13 (44396874 bp) chr Scaffold_14 (45393038 bp) chr Scaffold_15 (47938513 bp) chr Scaffold_16 (31980953 bp) chr Scaffold_17 (34923512 bp) chr Scaffold_18 (27737463 bp) Single-core mode: setting pid to 1 Paired-end alignments will be performed ======================================= The provided filenames for paired-end alignments are EPI-167_S10_L002_R1_001_val_1.fq.gz and EPI-167_S10_L002_R2_001_val_2.fq.gz Input files are in FastQ format Writing a C -> T converted version of the input file EPI-167_S10_L002_R1_001_val_1.fq.gz to EPI-167_S10_L002_R1_001_val_1.fq.gz_C_to_T.fastq slurmstepd: error: *** JOB 2268334 ON n2493 CANCELLED AT 2020-03-17T01:08:20 ***