/var/spool/slurm/d/job2268329/slurm_script: line 22: fg: no job control Path to Bowtie 2 specified as: bowtie2 Bowtie 2 seems to be working fine (tested command 'bowtie2 --version' [2.1.0]) Output format is BAM (default) Alignments will be written out in BAM format. Samtools found here: '/gscratch/srlab/programs/samtools-1.4/bin/samtools' Failed to move to /: No such file or directory USAGE: bismark [options] {-1 -2 | } [] (--help for more details) /var/spool/slurm/d/job2268329/slurm_script: line 48: -p: command not found *** Bismark methylation extractor version v0.21.0 *** Setting option '--no_overlap' since this is (normally) the right thing to do for paired-end data Summarising Bismark methylation extractor parameters: =============================================================== Bismark paired-end SAM format specified (default) Number of cores to be used: 14 Output will be written to the current directory ('/gscratch/scrubbed/strigg/analyses/20200316/TG_EPI-Test2') Summarising bedGraph parameters: =============================================================== Generating additional output in bedGraph and coverage format bedGraph format: coverage format: Using a cutoff of 1 read(s) to report cytosine positions Reporting and sorting cytosine methylation information in CpG context only (default) The bedGraph UNIX sort command will use the following memory setting: '75%'. Temporary directory used for sorting is the output directory Checking file >>*.bam<< for signs of file truncation... Captured error message: '[E::hts_open_format] Failed to open file *.bam' [ERROR] The file appears to be truncated, please ensure that there were no errors while copying the file!!! Exiting... Found no potential alignment reports in the current directory. Please specify a single Bismark alignment report file using the option '--alignment_report FILE' SYNOPSIS: This script uses a Bismark alignment report to generate a graphical HTML report page. Optionally, further reports of the Bismark suite such as deduplication, methylation extractor splitting or M-bias reports can be specified as well. If several Bismark reports are found in the same folder, a separate report will be generated for each of these, whereby the output filename will be derived from the Bismark alignment report file. bismark2report attempts to find optional reports automatically based on the file basename. USAGE: bismark2report [options] -o/--output Name of the output file (optional). If not specified explicitly, the output filename will be derived from the Bismark alignment report file. Specifying an output filename only works if the HTML report is to be generated for a single Bismark alignment report (and potentially additional reports). --dir Output directory. Output is written to the current directory if not specified explicitly. --alignment_report FILE If not specified explicitly, bismark2report attempts to find Bismark report file(s) in the current directory and produces a separate HTML report for each mapping report file. Based on the basename of the Bismark mapping report, bismark2report will also attempt to find the other Bismark reports (see below) for inclusion into the HTML report. Specifying a Bismark alignment report file is mandatory. --dedup_report FILE If not specified explicitly, bismark2report attempts to find a deduplication report file with the same basename as the Bismark mapping report (generated by deduplicate_bismark) in the current working directory. Including a deduplication report is optional, and using the FILE 'none' will skip this step entirely. --splitting_report FILE If not specified explicitly, bismark2report attempts to find a splitting report file with the same basename as the Bismark mapping report (generated by the Bismark methylation extractor) in the current working directory. Including a splitting report is optional, and using the FILE 'none' will skip this step entirely. --mbias_report FILE If not specified explicitly, bismark2report attempts to find a single M-bias report file with the same basename as the Bismark mapping report (generated by the Bismark methylation extractor) in the current working directory. Including an M-Bias report is optional, and using the FILE 'none' will skip this step entirely. --nucleotide_report FILE If not specified explicitly, bismark2report attempts to find a single nucleotide coverage report file with the same basename as the Bismark mapping report (generated by Bismark with the option '--nucleotide_coverage') in the current working directory. Including a nucleotide coverage statistics report is optional, and using the FILE 'none' will skip this report entirely. Script last modified: 07 August 2018 No Bismark/Bowtie2 single-end BAM files detected No Bismark/Bowtie2 paired-end BAM files detected No Bismark/HISAT2 single-end BAM files detected No Bismark/HISAT2 paired-end BAM files detected Error: No Bismark BAM files found to generate a Bismark project summary. Please respecify... USAGE: bismark2summary (*.bam), or bismark2summary --help for more information at /gscratch/srlab/programs/Bismark-0.21.0/bismark2summary line 201.