Output will be written into the directory: /gscratch/scrubbed/strigg/analyses/20190806_v074/ Processing paired-end Bismark output file(s) (SAM format): /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-205_S26_L004_R1_001_val_1_bismark_bt2_pe.bam /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-206_S27_L004_R1_001_val_1_bismark_bt2_pe.bam /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-214_S30_L004_R1_001_val_1_bismark_bt2_pe.bam /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-215_S31_L004_R1_001_val_1_bismark_bt2_pe.bam /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-220_S32_L004_R1_001_val_1_bismark_bt2_pe.bam /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-221_S33_L004_R1_001_val_1_bismark_bt2_pe.bam /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-226_S34_L004_R1_001_val_1_bismark_bt2_pe.bam /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-227_S35_L004_R1_001_val_1_bismark_bt2_pe.bam If there are several alignments to a single position in the genome the first alignment will be chosen. Since the input files are not in any way sorted this is a near-enough random selection of reads. Checking file >>/gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-205_S26_L004_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-205_S26_L004_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-205_S26_L004_R1_001_val_1_bismark_bt2_pe.bam: 7025362 Total number duplicated alignments removed: 1875252 (26.69%) Duplicated alignments were found at: 1025167 different position(s) Total count of deduplicated leftover sequences: 5150110 (73.31% of total) Checking file >>/gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-206_S27_L004_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-206_S27_L004_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-206_S27_L004_R1_001_val_1_bismark_bt2_pe.bam: 9918239 Total number duplicated alignments removed: 2423092 (24.43%) Duplicated alignments were found at: 1235195 different position(s) Total count of deduplicated leftover sequences: 7495147 (75.57% of total) Checking file >>/gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-214_S30_L004_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-214_S30_L004_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-214_S30_L004_R1_001_val_1_bismark_bt2_pe.bam: 13828640 Total number duplicated alignments removed: 3695743 (26.73%) Duplicated alignments were found at: 1989541 different position(s) Total count of deduplicated leftover sequences: 10132897 (73.27% of total) Checking file >>/gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-215_S31_L004_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-215_S31_L004_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-215_S31_L004_R1_001_val_1_bismark_bt2_pe.bam: 10236936 Total number duplicated alignments removed: 3030160 (29.60%) Duplicated alignments were found at: 1684943 different position(s) Total count of deduplicated leftover sequences: 7206776 (70.40% of total) Checking file >>/gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-220_S32_L004_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-220_S32_L004_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-220_S32_L004_R1_001_val_1_bismark_bt2_pe.bam: 7811982 Total number duplicated alignments removed: 2608881 (33.40%) Duplicated alignments were found at: 1463448 different position(s) Total count of deduplicated leftover sequences: 5203101 (66.60% of total) Checking file >>/gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-221_S33_L004_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-221_S33_L004_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-221_S33_L004_R1_001_val_1_bismark_bt2_pe.bam: 7892255 Total number duplicated alignments removed: 2308469 (29.25%) Duplicated alignments were found at: 1344013 different position(s) Total count of deduplicated leftover sequences: 5583786 (70.75% of total) Checking file >>/gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-226_S34_L004_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-226_S34_L004_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-226_S34_L004_R1_001_val_1_bismark_bt2_pe.bam: 5810273 Total number duplicated alignments removed: 1616015 (27.81%) Duplicated alignments were found at: 924266 different position(s) Total count of deduplicated leftover sequences: 4194258 (72.19% of total) Checking file >>/gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-227_S35_L004_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-227_S35_L004_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/scrubbed/strigg/analyses/20190806_v074/EPI-227_S35_L004_R1_001_val_1_bismark_bt2_pe.bam: 6848668 Total number duplicated alignments removed: 1928180 (28.15%) Duplicated alignments were found at: 1172653 different position(s) Total count of deduplicated leftover sequences: 4920488 (71.85% of total)