Bismark report for: /gscratch/scrubbed/strigg/Pgen_FQs/EPI-227_S35_L004_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/Pgen_FQs/EPI-227_S35_L004_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v074/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 13531234 Number of paired-end alignments with a unique best hit: 6848677 Mapping efficiency: 50.6% Sequence pairs with no alignments under any condition: 5415876 Sequence pairs did not map uniquely: 1266681 Sequence pairs which were discarded because genomic sequence could not be extracted: 9 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3353489 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 3495179 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 209732703 Total methylated C's in CpG context: 7552418 Total methylated C's in CHG context: 1267634 Total methylated C's in CHH context: 8547890 Total methylated C's in Unknown context: 313577 Total unmethylated C's in CpG context: 21092550 Total unmethylated C's in CHG context: 43317740 Total unmethylated C's in CHH context: 127954471 Total unmethylated C's in Unknown context: 1377183 C methylated in CpG context: 26.4% C methylated in CHG context: 2.8% C methylated in CHH context: 6.3% C methylated in unknown context (CN or CHN): 18.5% Bismark completed in 0d 1h 6m 45s