Bismark report for: /gscratch/scrubbed/strigg/Pgen_FQs/EPI-220_S32_L004_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/Pgen_FQs/EPI-220_S32_L004_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v074/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 15670592 Number of paired-end alignments with a unique best hit: 7812000 Mapping efficiency: 49.9% Sequence pairs with no alignments under any condition: 6437579 Sequence pairs did not map uniquely: 1421013 Sequence pairs which were discarded because genomic sequence could not be extracted: 18 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3807899 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 4004083 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 241180813 Total methylated C's in CpG context: 8360975 Total methylated C's in CHG context: 1444434 Total methylated C's in CHH context: 9329429 Total methylated C's in Unknown context: 348452 Total unmethylated C's in CpG context: 25279896 Total unmethylated C's in CHG context: 50350749 Total unmethylated C's in CHH context: 146415330 Total unmethylated C's in Unknown context: 1649919 C methylated in CpG context: 24.9% C methylated in CHG context: 2.8% C methylated in CHH context: 6.0% C methylated in unknown context (CN or CHN): 17.4% Bismark completed in 0d 1h 16m 45s