Output will be written into the directory: /gscratch/srlab/strigg/analyses/20190415_10K/ Processing paired-end Bismark output file(s) (SAM format): /gscratch/srlab/strigg/analyses/20190415_10K/Tank2-025-026_R1_001_bismark_bt2_pe.bam /gscratch/srlab/strigg/analyses/20190415_10K/Tank2-025-026_trim_R1_001_bismark_bt2_pe.bam /gscratch/srlab/strigg/analyses/20190415_10K/Tank3-15-16_R1_001_bismark_bt2_pe.bam /gscratch/srlab/strigg/analyses/20190415_10K/Tank3-15-16_trim_R1_001_bismark_bt2_pe.bam If there are several alignments to a single position in the genome the first alignment will be chosen. Since the input files are not in any way sorted this is a near-enough random selection of reads. Checking file >>/gscratch/srlab/strigg/analyses/20190415_10K/Tank2-025-026_R1_001_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: Tank2-025-026_R1_001_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/srlab/strigg/analyses/20190415_10K/Tank2-025-026_R1_001_bismark_bt2_pe.bam: 44222014 Total number duplicated alignments removed: 4089205 (9.25%) Duplicated alignments were found at: 3434427 different position(s) Total count of deduplicated leftover sequences: 40132809 (90.75% of total) Checking file >>/gscratch/srlab/strigg/analyses/20190415_10K/Tank2-025-026_trim_R1_001_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: Tank2-025-026_trim_R1_001_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/srlab/strigg/analyses/20190415_10K/Tank2-025-026_trim_R1_001_bismark_bt2_pe.bam: 42892839 Total number duplicated alignments removed: 3932820 (9.17%) Duplicated alignments were found at: 3410251 different position(s) Total count of deduplicated leftover sequences: 38960019 (90.83% of total) Checking file >>/gscratch/srlab/strigg/analyses/20190415_10K/Tank3-15-16_R1_001_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: Tank3-15-16_R1_001_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/srlab/strigg/analyses/20190415_10K/Tank3-15-16_R1_001_bismark_bt2_pe.bam: 32660140 Total number duplicated alignments removed: 2547287 (7.80%) Duplicated alignments were found at: 2151243 different position(s) Total count of deduplicated leftover sequences: 30112853 (92.20% of total) Checking file >>/gscratch/srlab/strigg/analyses/20190415_10K/Tank3-15-16_trim_R1_001_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: Tank3-15-16_trim_R1_001_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/srlab/strigg/analyses/20190415_10K/Tank3-15-16_trim_R1_001_bismark_bt2_pe.bam: 31686658 Total number duplicated alignments removed: 2444863 (7.72%) Duplicated alignments were found at: 2140207 different position(s) Total count of deduplicated leftover sequences: 29241795 (92.28% of total)