Output will be written into the directory: /gscratch/srlab/strigg/analyses/20190214_30K/ Processing paired-end Bismark output file(s) (SAM format): /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.bam /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.bam /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.bam /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.bam If there are several alignments to a single position in the genome the first alignment will be chosen. Since the input files are not in any way sorted this is a near-enough random selection of reads. Checking file >>/gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.bam: 15600077 Total number duplicated alignments removed: 2723291 (17.46%) Duplicated alignments were found at: 1171198 different position(s) Total count of deduplicated leftover sequences: 12876786 (82.54% of total) Checking file >>/gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.bam: 14908234 Total number duplicated alignments removed: 2626788 (17.62%) Duplicated alignments were found at: 1117697 different position(s) Total count of deduplicated leftover sequences: 12281446 (82.38% of total) Checking file >>/gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.bam: 15855321 Total number duplicated alignments removed: 2815636 (17.76%) Duplicated alignments were found at: 1216468 different position(s) Total count of deduplicated leftover sequences: 13039685 (82.24% of total) Checking file >>/gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.bam: 14219508 Total number duplicated alignments removed: 2412714 (16.97%) Duplicated alignments were found at: 1047169 different position(s) Total count of deduplicated leftover sequences: 11806794 (83.03% of total)