*** Bismark methylation extractor version v0.19.0 *** Setting option '--no_overlap' since this is (normally) the right thing to do for paired-end data Core usage currently set to more than 20 threads. Let's see how this goes... (set value: 28) Summarising Bismark methylation extractor parameters: =============================================================== Bismark paired-end SAM format specified (default) Number of cores to be used: 28 Output path specified as: /gscratch/srlab/strigg/analyses/20190214_30K/ Summarising bedGraph parameters: =============================================================== Generating additional output in bedGraph and coverage format bedGraph format: coverage format: Using a cutoff of 1 read(s) to report cytosine positions Reporting and sorting cytosine methylation information in CpG context only (default) The bedGraph UNIX sort command will use the following memory setting: '2G'. Temporary directory used for sorting is the output directory Checking file >>/gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... ...passed! Writing result file containing methylation information for C in CpG context from the original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:PGA_scaffold1__77_contigs__length_89643857 LN:89643857 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold64742__1_contigs__length_65506 LN:65506 skipping SAM header line: @SQ SN:PGA_scaffold64773__1_contigs__length_73184 LN:73184 skipping SAM header line: @SQ SN:PGA_scaffold64789__1_contigs__length_47691 LN:47691 skipping SAM header line: @SQ SN:PGA_scaffold64790__1_contigs__length_34222 LN:34222 skipping SAM header line: @SQ SN:PGA_scaffold64825__1_contigs__length_54777 LN:54777 skipping SAM header line: @SQ SN:PGA_scaffold64826__1_contigs__length_30214 LN:30214 skipping SAM header line: @SQ SN:PGA_scaffold64882__1_contigs__length_39867 LN:39867 skipping SAM header line: @SQ SN:PGA_scaffold64903__1_contigs__length_42771 LN:42771 skipping SAM header line: @SQ SN:PGA_scaffold64931__1_contigs__length_44617 LN:44617 skipping SAM header line: @SQ SN:PGA_scaffold64934__1_contigs__length_30286 LN:30286 skipping SAM header line: @SQ SN:PGA_scaffold64954__1_contigs__length_35731 LN:35731 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold65174__1_contigs__length_38341 LN:38341 skipping SAM header line: @SQ SN:PGA_scaffold65186__1_contigs__length_41412 LN:41412 skipping SAM header line: @SQ SN:PGA_scaffold65198__1_contigs__length_44989 LN:44989 skipping SAM header line: @SQ SN:PGA_scaffold65255__1_contigs__length_59866 LN:59866 skipping SAM header line: @SQ SN:PGA_scaffold65278__1_contigs__length_62003 LN:62003 skipping SAM header line: @SQ SN:PGA_scaffold65284__1_contigs__length_50840 LN:50840 skipping SAM header line: @SQ SN:PGA_scaffold65305__1_contigs__length_40067 LN:40067 skipping SAM header line: @SQ SN:PGA_scaffold65307__1_contigs__length_57187 LN:57187 skipping SAM header line: @SQ SN:PGA_scaffold65313__1_contigs__length_38893 LN:38893 skipping SAM header line: @SQ SN:PGA_scaffold65329__1_contigs__length_30096 LN:30096 skipping SAM header line: @SQ SN:PGA_scaffold65356__1_contigs__length_44587 LN:44587 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold65781__1_contigs__length_42302 LN:42302 skipping SAM header line: @SQ SN:PGA_scaffold65786__1_contigs__length_56597 LN:56597 skipping SAM header line: @SQ SN:PGA_scaffold65807__1_contigs__length_35379 LN:35379 skipping SAM header line: @SQ SN:PGA_scaffold65809__1_contigs__length_36632 LN:36632 skipping SAM header line: @SQ SN:PGA_scaffold65815__1_contigs__length_49308 LN:49308 skipping SAM header line: @SQ SN:PGA_scaffold65846__1_contigs__length_33986 LN:33986 skipping SAM header line: @SQ SN:PGA_scaffold65851__1_contigs__length_44621 LN:44621 skipping SAM header line: @SQ SN:PGA_scaffold65866__1_contigs__length_90471 LN:90471 skipping SAM header line: @SQ SN:PGA_scaffold65911__1_contigs__length_48303 LN:48303 skipping SAM header line: @SQ SN:PGA_scaffold65912__1_contigs__length_34561 LN:34561 skipping SAM header line: @SQ SN:PGA_scaffold65945__1_contigs__length_40559 LN:40559 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold66173__1_contigs__length_34319 LN:34319 skipping SAM header line: @SQ SN:PGA_scaffold66178__1_contigs__length_49601 LN:49601 skipping SAM header line: @SQ SN:PGA_scaffold66186__1_contigs__length_45928 LN:45928 skipping SAM header line: @SQ SN:PGA_scaffold66196__1_contigs__length_53841 LN:53841 skipping SAM header line: @SQ SN:PGA_scaffold66212__1_contigs__length_32503 LN:32503 skipping SAM header line: @SQ SN:PGA_scaffold66276__1_contigs__length_39820 LN:39820 skipping SAM header line: @SQ SN:PGA_scaffold66293__1_contigs__length_33304 LN:33304 skipping SAM header line: @SQ SN:PGA_scaffold66318__1_contigs__length_45619 LN:45619 skipping SAM header line: @SQ SN:PGA_scaffold66338__1_contigs__length_30756 LN:30756 skipping SAM header line: @SQ SN:PGA_scaffold66399__1_contigs__length_42456 LN:42456 skipping SAM header line: @SQ SN:PGA_scaffold66419__1_contigs__length_49715 LN:49715 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold69479__1_contigs__length_32808 LN:32808 skipping SAM header line: @SQ SN:PGA_scaffold69484__1_contigs__length_31150 LN:31150 skipping SAM header line: @SQ SN:PGA_scaffold69495__1_contigs__length_53052 LN:53052 skipping SAM header line: @SQ SN:PGA_scaffold69529__1_contigs__length_33639 LN:33639 skipping SAM header line: @SQ SN:PGA_scaffold69555__1_contigs__length_42966 LN:42966 skipping SAM header line: @SQ SN:PGA_scaffold69556__1_contigs__length_33961 LN:33961 skipping SAM header line: @SQ SN:PGA_scaffold69619__1_contigs__length_30388 LN:30388 skipping SAM header line: @SQ SN:PGA_scaffold69621__1_contigs__length_30422 LN:30422 skipping SAM header line: @SQ SN:PGA_scaffold69622__1_contigs__length_37660 LN:37660 skipping SAM header line: @SQ SN:PGA_scaffold69631__1_contigs__length_44909 LN:44909 skipping SAM header line: @SQ SN:PGA_scaffold69645__1_contigs__length_46339 LN:46339 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold69901__1_contigs__length_33452 LN:33452 skipping SAM header line: @SQ SN:PGA_scaffold69924__1_contigs__length_44446 LN:44446 skipping SAM header line: @SQ SN:PGA_scaffold69935__1_contigs__length_32162 LN:32162 skipping SAM header line: @SQ SN:PGA_scaffold69949__1_contigs__length_34320 LN:34320 skipping SAM header line: @SQ SN:PGA_scaffold69958__1_contigs__length_74035 LN:74035 skipping SAM header line: @SQ SN:PGA_scaffold69967__1_contigs__length_33074 LN:33074 skipping SAM header line: @SQ SN:PGA_scaffold69970__1_contigs__length_36785 LN:36785 skipping SAM header line: @SQ SN:PGA_scaffold69984__1_contigs__length_60451 LN:60451 skipping SAM header line: @SQ SN:PGA_scaffold70035__1_contigs__length_36658 LN:36658 skipping SAM header line: @SQ SN:PGA_scaffold70070__1_contigs__length_49697 LN:49697 skipping SAM header line: @SQ SN:PGA_scaffold70086__1_contigs__length_42850 LN:42850 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold70287__1_contigs__length_50534 LN:50534 skipping SAM header line: @SQ SN:PGA_scaffold70301__1_contigs__length_33670 LN:33670 skipping SAM header line: @SQ SN:PGA_scaffold70359__1_contigs__length_31823 LN:31823 skipping SAM header line: @SQ SN:PGA_scaffold70409__1_contigs__length_30379 LN:30379 skipping SAM header line: @SQ SN:PGA_scaffold70448__1_contigs__length_39500 LN:39500 skipping SAM header line: @SQ SN:PGA_scaffold70468__1_contigs__length_51685 LN:51685 skipping SAM header line: @SQ SN:PGA_scaffold70474__1_contigs__length_50365 LN:50365 skipping SAM header line: @SQ SN:PGA_scaffold70476__1_contigs__length_49360 LN:49360 skipping SAM header line: @SQ SN:PGA_scaffold70489__1_contigs__length_43424 LN:43424 skipping SAM header line: @SQ SN:PGA_scaffold70492__1_contigs__length_45967 LN:45967 skipping SAM header line: @SQ SN:PGA_scaffold70506__1_contigs__length_45721 LN:45721 skipping SAM header line: @SQ 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SN:PGA_scaffold313649__2_contigs__length_2716785 LN:2716785 skipping SAM header line: @PG ID:Bismark VN:v0.19.0 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64 --samtools_path /gscratch/srlab/programs/samtools-1.9 --score_min L,0,-1.2 -I 60 -p 28 --genome /gscratch/srlab/strigg/data/Pgenr/Pgenr_070_30K/ -1 /gscratch/srlab/strigg/data/Pgenr/FASTQS/data_for_genome_comparison/EPI-111_S29_L005_R1_001_val_1.fq.gz -2 /gscratch/srlab/strigg/data/Pgenr/FASTQS/data_for_genome_comparison/EPI-111_S29_L005_R2_001_val_2.fq.gz -o /gscratch/srlab/strigg/analyses/20190214_30K" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed 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Exiting.. Finished processing child process. Exiting.. Now waiting for all child processes to complete Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Finished processing child process. Exiting.. Processed lines: 12500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 12500000 Processed lines: 12500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Merging individual splitting reports into overall report: '/gscratch/srlab/strigg/analyses/20190214_30K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: Processed 12876786 lines in total Total number of methylation call strings processed: 25753572 Final Cytosine Methylation Report ================================= Total number of C's analysed: 291449034 Total methylated C's in CpG context: 10034961 Total methylated C's in CHG context: 1469857 Total methylated C's in CHH context: 3341240 Total C to T conversions in CpG context: 29496188 Total C to T conversions in CHG context: 58867342 Total C to T conversions in CHH context: 188239446 C methylated in CpG context: 25.4% C methylated in CHG context: 2.4% C methylated in CHH context: 1.7% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 101 Maximum read length of Read 2: 99 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 101 Maximum read length of Read 2: 99 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >/gscratch/srlab/strigg/analyses/20190214_30K/< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 2G Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: Writing bedGraph to file: EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Changed directory to /gscratch/srlab/strigg/analyses/20190214_30K/ The genome of interest was specified to contain gazillions of chromosomes or scaffolds. Merging all input files and sorting everything in memory instead of writing out individual chromosome files... Writing all merged methylation calls to temp file EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz.methylation_calls.merged Finished writing methylation calls from CpG_OT_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt to merged temp file Finished writing methylation calls from CpG_OB_EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt to merged temp file Sorting input file EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz.methylation_calls.merged by positions (using -S of 2G) Successfully deleted the temporary input file EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz.methylation_calls.merged Finished BedGraph conversion ... Checking file >>/gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... ...passed! Writing result file containing methylation information for C in CpG context from the original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:PGA_scaffold1__77_contigs__length_89643857 LN:89643857 skipping SAM header line: @SQ SN:PGA_scaffold2__36_contigs__length_69596280 LN:69596280 skipping SAM header line: @SQ SN:PGA_scaffold3__111_contigs__length_57743597 LN:57743597 skipping SAM header line: @SQ SN:PGA_scaffold4__129_contigs__length_65288255 LN:65288255 skipping SAM header line: @SQ SN:PGA_scaffold5__109_contigs__length_67248332 LN:67248332 skipping SAM header line: @SQ SN:PGA_scaffold6__104_contigs__length_61759565 LN:61759565 skipping SAM header line: @SQ SN:PGA_scaffold7__69_contigs__length_43120122 LN:43120122 skipping SAM header line: @SQ SN:PGA_scaffold8__63_contigs__length_61151155 LN:61151155 skipping SAM header line: @SQ SN:PGA_scaffold9__45_contigs__length_38581958 LN:38581958 skipping SAM header line: @SQ SN:PGA_scaffold10__49_contigs__length_53961475 LN:53961475 skipping SAM header line: @SQ SN:PGA_scaffold11__79_contigs__length_51449921 LN:51449921 skipping SAM header line: @SQ SN:PGA_scaffold12__71_contigs__length_50438331 LN:50438331 skipping SAM header line: @SQ SN:PGA_scaffold13__52_contigs__length_44396874 LN:44396874 skipping SAM header line: @SQ SN:PGA_scaffold14__91_contigs__length_45393038 LN:45393038 skipping SAM header line: @SQ SN:PGA_scaffold15__101_contigs__length_47938513 LN:47938513 skipping SAM header line: @SQ SN:PGA_scaffold16__33_contigs__length_31980953 LN:31980953 skipping SAM header line: @SQ SN:PGA_scaffold17__51_contigs__length_34923512 LN:34923512 skipping SAM header line: @SQ SN:PGA_scaffold18__69_contigs__length_27737463 LN:27737463 skipping SAM header line: @SQ SN:PGA_scaffold100__1_contigs__length_37850 LN:37850 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold55378__1_contigs__length_34882 LN:34882 skipping SAM header line: @SQ SN:PGA_scaffold55381__1_contigs__length_30600 LN:30600 skipping SAM header line: @SQ SN:PGA_scaffold55711__1_contigs__length_39787 LN:39787 skipping SAM header line: @SQ SN:PGA_scaffold55714__1_contigs__length_36372 LN:36372 skipping SAM header line: @SQ SN:PGA_scaffold55745__1_contigs__length_39718 LN:39718 skipping SAM header line: @SQ SN:PGA_scaffold55747__1_contigs__length_42692 LN:42692 skipping SAM header line: @SQ SN:PGA_scaffold55855__1_contigs__length_34489 LN:34489 skipping SAM header line: @SQ SN:PGA_scaffold55860__1_contigs__length_50662 LN:50662 skipping SAM header line: @SQ SN:PGA_scaffold55979__1_contigs__length_33616 LN:33616 skipping SAM header line: @SQ SN:PGA_scaffold56091__1_contigs__length_39544 LN:39544 skipping SAM header line: @SQ SN:PGA_scaffold56365__1_contigs__length_56315 LN:56315 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold57070__1_contigs__length_35859 LN:35859 skipping SAM header line: @SQ SN:PGA_scaffold57215__1_contigs__length_43048 LN:43048 skipping SAM header line: @SQ SN:PGA_scaffold57298__1_contigs__length_31957 LN:31957 skipping SAM header line: @SQ SN:PGA_scaffold57301__1_contigs__length_34452 LN:34452 skipping SAM header line: @SQ SN:PGA_scaffold57398__1_contigs__length_30770 LN:30770 skipping SAM header line: @SQ SN:PGA_scaffold57419__1_contigs__length_56657 LN:56657 skipping SAM header line: @SQ SN:PGA_scaffold57435__1_contigs__length_39348 LN:39348 skipping SAM header line: @SQ SN:PGA_scaffold57479__1_contigs__length_34907 LN:34907 skipping SAM header line: @SQ SN:PGA_scaffold57490__1_contigs__length_31663 LN:31663 skipping SAM header line: @SQ SN:PGA_scaffold57492__1_contigs__length_41689 LN:41689 skipping SAM header line: @SQ SN:PGA_scaffold57510__1_contigs__length_39178 LN:39178 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold57901__1_contigs__length_33405 LN:33405 skipping SAM header line: @SQ SN:PGA_scaffold58076__1_contigs__length_32943 LN:32943 skipping SAM header line: @SQ SN:PGA_scaffold58238__1_contigs__length_68373 LN:68373 skipping SAM header line: @SQ SN:PGA_scaffold58264__1_contigs__length_33661 LN:33661 skipping SAM header line: @SQ SN:PGA_scaffold58284__1_contigs__length_33826 LN:33826 skipping SAM header line: @SQ SN:PGA_scaffold58361__1_contigs__length_52751 LN:52751 skipping SAM header line: @SQ SN:PGA_scaffold58366__1_contigs__length_37326 LN:37326 skipping SAM header line: @SQ SN:PGA_scaffold58382__1_contigs__length_41713 LN:41713 skipping SAM header line: @SQ SN:PGA_scaffold58383__1_contigs__length_44366 LN:44366 skipping SAM header line: @SQ SN:PGA_scaffold58388__1_contigs__length_32850 LN:32850 skipping SAM header line: @SQ SN:PGA_scaffold58393__1_contigs__length_33434 LN:33434 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold58676__1_contigs__length_42740 LN:42740 skipping SAM header line: @SQ SN:PGA_scaffold58700__1_contigs__length_38161 LN:38161 skipping SAM header line: @SQ SN:PGA_scaffold58712__1_contigs__length_43972 LN:43972 skipping SAM header line: @SQ SN:PGA_scaffold58749__1_contigs__length_42553 LN:42553 skipping SAM header line: @SQ SN:PGA_scaffold58976__1_contigs__length_32077 LN:32077 skipping SAM header line: @SQ SN:PGA_scaffold58984__1_contigs__length_30766 LN:30766 skipping SAM header line: @SQ SN:PGA_scaffold59041__1_contigs__length_49211 LN:49211 skipping SAM header line: @SQ SN:PGA_scaffold59054__1_contigs__length_30586 LN:30586 skipping SAM header line: @SQ SN:PGA_scaffold59056__1_contigs__length_33360 LN:33360 skipping SAM header line: @SQ SN:PGA_scaffold59166__1_contigs__length_30875 LN:30875 skipping SAM header line: @SQ SN:PGA_scaffold59171__1_contigs__length_43122 LN:43122 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold59687__1_contigs__length_34469 LN:34469 skipping SAM header line: @SQ SN:PGA_scaffold59721__1_contigs__length_42511 LN:42511 skipping SAM header line: @SQ SN:PGA_scaffold59723__1_contigs__length_39806 LN:39806 skipping SAM header line: @SQ SN:PGA_scaffold59727__1_contigs__length_31211 LN:31211 skipping SAM header line: @SQ SN:PGA_scaffold59729__1_contigs__length_32301 LN:32301 skipping SAM header line: @SQ SN:PGA_scaffold59750__1_contigs__length_36894 LN:36894 skipping SAM header line: @SQ SN:PGA_scaffold59858__1_contigs__length_39712 LN:39712 skipping SAM header line: @SQ SN:PGA_scaffold59872__1_contigs__length_51346 LN:51346 skipping SAM header line: @SQ SN:PGA_scaffold59907__1_contigs__length_30865 LN:30865 skipping SAM header line: @SQ SN:PGA_scaffold59933__1_contigs__length_43116 LN:43116 skipping SAM header line: @SQ SN:PGA_scaffold59943__1_contigs__length_33685 LN:33685 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold60318__1_contigs__length_36247 LN:36247 skipping SAM header line: @SQ SN:PGA_scaffold60412__1_contigs__length_79597 LN:79597 skipping SAM header line: @SQ SN:PGA_scaffold60413__1_contigs__length_42267 LN:42267 skipping SAM header line: @SQ SN:PGA_scaffold60414__1_contigs__length_49107 LN:49107 skipping SAM header line: @SQ SN:PGA_scaffold60441__1_contigs__length_43835 LN:43835 skipping SAM header line: @SQ SN:PGA_scaffold60488__1_contigs__length_50761 LN:50761 skipping SAM header line: @SQ SN:PGA_scaffold60490__1_contigs__length_43435 LN:43435 skipping SAM header line: @SQ SN:PGA_scaffold60615__1_contigs__length_40286 LN:40286 skipping SAM header line: @SQ SN:PGA_scaffold60652__1_contigs__length_52273 LN:52273 skipping SAM header line: @SQ SN:PGA_scaffold60657__1_contigs__length_45209 LN:45209 skipping SAM header line: @SQ SN:PGA_scaffold60699__1_contigs__length_33841 LN:33841 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold62542__1_contigs__length_32921 LN:32921 skipping SAM header line: @SQ SN:PGA_scaffold62544__1_contigs__length_41829 LN:41829 skipping SAM header line: @SQ SN:PGA_scaffold62591__1_contigs__length_64464 LN:64464 skipping SAM header line: @SQ SN:PGA_scaffold62601__1_contigs__length_36053 LN:36053 skipping SAM header line: @SQ SN:PGA_scaffold62602__1_contigs__length_30153 LN:30153 skipping SAM header line: @SQ SN:PGA_scaffold62661__1_contigs__length_34648 LN:34648 skipping SAM header line: @SQ SN:PGA_scaffold62687__1_contigs__length_56019 LN:56019 skipping SAM header line: @SQ SN:PGA_scaffold62752__1_contigs__length_33202 LN:33202 skipping SAM header line: @SQ SN:PGA_scaffold62854__1_contigs__length_47148 LN:47148 skipping SAM header line: @SQ SN:PGA_scaffold62862__1_contigs__length_31728 LN:31728 skipping SAM header line: @SQ SN:PGA_scaffold62865__1_contigs__length_39731 LN:39731 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold62982__1_contigs__length_51892 LN:51892 skipping SAM header line: @SQ SN:PGA_scaffold63008__1_contigs__length_40245 LN:40245 skipping SAM header line: @SQ SN:PGA_scaffold63022__1_contigs__length_31832 LN:31832 skipping SAM header line: @SQ SN:PGA_scaffold63033__1_contigs__length_64137 LN:64137 skipping SAM header line: @SQ SN:PGA_scaffold63057__1_contigs__length_30046 LN:30046 skipping SAM header line: @SQ SN:PGA_scaffold63059__1_contigs__length_30993 LN:30993 skipping SAM header line: @SQ SN:PGA_scaffold63073__1_contigs__length_59843 LN:59843 skipping SAM header line: @SQ SN:PGA_scaffold63076__1_contigs__length_65566 LN:65566 skipping SAM header line: @SQ SN:PGA_scaffold63085__1_contigs__length_46291 LN:46291 skipping SAM header line: @SQ SN:PGA_scaffold63090__1_contigs__length_43963 LN:43963 skipping SAM header line: @SQ SN:PGA_scaffold63101__1_contigs__length_45043 LN:45043 skipping SAM header line: @SQ SN:PGA_scaffold63115__1_contigs__length_32288 LN:32288 skipping SAM header line: @SQ SN:PGA_scaffold63140__1_contigs__length_32392 LN:32392 skipping SAM header line: @SQ SN:PGA_scaffold63144__1_contigs__length_70359 LN:70359 skipping SAM header line: @SQ SN:PGA_scaffold63154__1_contigs__length_40784 LN:40784 skipping SAM header line: @SQ SN:PGA_scaffold63158__1_contigs__length_32781 LN:32781 skipping SAM header line: @SQ SN:PGA_scaffold63175__1_contigs__length_32817 LN:32817 skipping SAM header line: @SQ SN:PGA_scaffold63196__1_contigs__length_54760 LN:54760 skipping SAM header line: @SQ SN:PGA_scaffold63198__1_contigs__length_39852 LN:39852 skipping SAM header line: @SQ SN:PGA_scaffold63251__1_contigs__length_76351 LN:76351 skipping SAM header line: @SQ SN:PGA_scaffold63277__1_contigs__length_43210 LN:43210 skipping SAM header line: @SQ SN:PGA_scaffold63280__1_contigs__length_78184 LN:78184 skipping SAM header line: @SQ SN:PGA_scaffold63282__1_contigs__length_48451 LN:48451 skipping SAM header line: @SQ SN:PGA_scaffold63286__1_contigs__length_64799 LN:64799 skipping SAM header line: @SQ SN:PGA_scaffold63295__1_contigs__length_73242 LN:73242 skipping SAM header line: @SQ SN:PGA_scaffold63327__1_contigs__length_41823 LN:41823 skipping SAM header line: @SQ SN:PGA_scaffold63330__1_contigs__length_32542 LN:32542 skipping SAM header line: @SQ SN:PGA_scaffold63338__1_contigs__length_30825 LN:30825 skipping SAM header line: @SQ SN:PGA_scaffold63480__1_contigs__length_39812 LN:39812 skipping SAM header line: @SQ SN:PGA_scaffold63504__1_contigs__length_83511 LN:83511 skipping SAM header line: @SQ SN:PGA_scaffold63543__1_contigs__length_67322 LN:67322 skipping SAM header line: @SQ SN:PGA_scaffold63594__1_contigs__length_42878 LN:42878 skipping SAM header line: @SQ SN:PGA_scaffold63635__1_contigs__length_31389 LN:31389 skipping SAM header line: @SQ SN:PGA_scaffold63681__1_contigs__length_35508 LN:35508 skipping SAM header line: @SQ SN:PGA_scaffold63729__1_contigs__length_32731 LN:32731 skipping SAM header line: @SQ SN:PGA_scaffold63732__1_contigs__length_50829 LN:50829 skipping SAM header line: @SQ SN:PGA_scaffold63734__1_contigs__length_31184 LN:31184 skipping SAM header line: @SQ SN:PGA_scaffold63741__1_contigs__length_36520 LN:36520 skipping SAM header line: @SQ SN:PGA_scaffold63748__1_contigs__length_33842 LN:33842 skipping SAM header line: @SQ SN:PGA_scaffold63749__1_contigs__length_42782 LN:42782 skipping SAM header line: @SQ SN:PGA_scaffold63751__1_contigs__length_41022 LN:41022 skipping SAM header line: @SQ SN:PGA_scaffold63753__1_contigs__length_33280 LN:33280 skipping SAM header line: @SQ SN:PGA_scaffold63754__1_contigs__length_42061 LN:42061 skipping SAM header line: @SQ SN:PGA_scaffold63762__1_contigs__length_32202 LN:32202 skipping SAM header line: @SQ SN:PGA_scaffold63788__1_contigs__length_79173 LN:79173 skipping SAM header line: @SQ SN:PGA_scaffold63874__1_contigs__length_36908 LN:36908 skipping SAM header line: @SQ SN:PGA_scaffold63883__1_contigs__length_68258 LN:68258 skipping SAM header line: @SQ SN:PGA_scaffold63886__1_contigs__length_33964 LN:33964 skipping SAM header line: @SQ SN:PGA_scaffold63901__1_contigs__length_33737 LN:33737 skipping SAM header line: @SQ SN:PGA_scaffold63902__1_contigs__length_30981 LN:30981 skipping SAM header line: @SQ SN:PGA_scaffold63904__1_contigs__length_56940 LN:56940 skipping SAM header line: @SQ SN:PGA_scaffold63956__1_contigs__length_32393 LN:32393 skipping SAM header line: @SQ SN:PGA_scaffold63976__1_contigs__length_45677 LN:45677 skipping SAM header line: @SQ SN:PGA_scaffold63999__1_contigs__length_42041 LN:42041 skipping SAM header line: @SQ SN:PGA_scaffold64025__1_contigs__length_40125 LN:40125 skipping SAM header line: @SQ SN:PGA_scaffold64049__1_contigs__length_45875 LN:45875 skipping SAM header line: @SQ SN:PGA_scaffold64054__1_contigs__length_70679 LN:70679 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold64383__1_contigs__length_88001 LN:88001 skipping SAM header line: @SQ SN:PGA_scaffold64394__1_contigs__length_33404 LN:33404 skipping SAM header line: @SQ SN:PGA_scaffold64426__1_contigs__length_49077 LN:49077 skipping SAM header line: @SQ SN:PGA_scaffold64456__1_contigs__length_38607 LN:38607 skipping SAM header line: @SQ SN:PGA_scaffold64462__1_contigs__length_30068 LN:30068 skipping SAM header line: @SQ SN:PGA_scaffold64465__1_contigs__length_42085 LN:42085 skipping SAM header line: @SQ SN:PGA_scaffold64466__1_contigs__length_38330 LN:38330 skipping SAM header line: @SQ SN:PGA_scaffold64470__1_contigs__length_48847 LN:48847 skipping SAM header line: @SQ SN:PGA_scaffold64471__1_contigs__length_34271 LN:34271 skipping SAM header line: @SQ SN:PGA_scaffold64473__1_contigs__length_33437 LN:33437 skipping SAM header line: @SQ SN:PGA_scaffold64529__1_contigs__length_33564 LN:33564 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold64699__1_contigs__length_32242 LN:32242 skipping SAM header line: @SQ SN:PGA_scaffold64701__1_contigs__length_57865 LN:57865 skipping SAM header line: @SQ SN:PGA_scaffold64702__1_contigs__length_30546 LN:30546 skipping SAM header line: @SQ SN:PGA_scaffold64737__1_contigs__length_40853 LN:40853 skipping SAM header line: @SQ SN:PGA_scaffold64742__1_contigs__length_65506 LN:65506 skipping SAM header line: @SQ SN:PGA_scaffold64773__1_contigs__length_73184 LN:73184 skipping SAM header line: @SQ SN:PGA_scaffold64789__1_contigs__length_47691 LN:47691 skipping SAM header line: @SQ SN:PGA_scaffold64790__1_contigs__length_34222 LN:34222 skipping SAM header line: @SQ SN:PGA_scaffold64825__1_contigs__length_54777 LN:54777 skipping SAM header line: @SQ SN:PGA_scaffold64826__1_contigs__length_30214 LN:30214 skipping SAM header line: @SQ SN:PGA_scaffold64882__1_contigs__length_39867 LN:39867 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold65091__1_contigs__length_44371 LN:44371 skipping SAM header line: @SQ SN:PGA_scaffold65116__1_contigs__length_38844 LN:38844 skipping SAM header line: @SQ SN:PGA_scaffold65119__1_contigs__length_57101 LN:57101 skipping SAM header line: @SQ SN:PGA_scaffold65171__1_contigs__length_46480 LN:46480 skipping SAM header line: @SQ SN:PGA_scaffold65174__1_contigs__length_38341 LN:38341 skipping SAM header line: @SQ SN:PGA_scaffold65186__1_contigs__length_41412 LN:41412 skipping SAM header line: @SQ SN:PGA_scaffold65198__1_contigs__length_44989 LN:44989 skipping SAM header line: @SQ SN:PGA_scaffold65255__1_contigs__length_59866 LN:59866 skipping SAM header line: @SQ SN:PGA_scaffold65278__1_contigs__length_62003 LN:62003 skipping SAM header line: @SQ SN:PGA_scaffold65284__1_contigs__length_50840 LN:50840 skipping SAM header line: @SQ SN:PGA_scaffold65305__1_contigs__length_40067 LN:40067 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold65693__1_contigs__length_34648 LN:34648 skipping SAM header line: @SQ SN:PGA_scaffold65737__1_contigs__length_37231 LN:37231 skipping SAM header line: @SQ SN:PGA_scaffold65765__1_contigs__length_39217 LN:39217 skipping SAM header line: @SQ SN:PGA_scaffold65777__1_contigs__length_32724 LN:32724 skipping SAM header line: @SQ SN:PGA_scaffold65781__1_contigs__length_42302 LN:42302 skipping SAM header line: @SQ SN:PGA_scaffold65786__1_contigs__length_56597 LN:56597 skipping SAM header line: @SQ SN:PGA_scaffold65807__1_contigs__length_35379 LN:35379 skipping SAM header line: @SQ SN:PGA_scaffold65809__1_contigs__length_36632 LN:36632 skipping SAM header line: @SQ SN:PGA_scaffold65815__1_contigs__length_49308 LN:49308 skipping SAM header line: @SQ SN:PGA_scaffold65846__1_contigs__length_33986 LN:33986 skipping SAM header line: @SQ SN:PGA_scaffold65851__1_contigs__length_44621 LN:44621 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold66079__1_contigs__length_37644 LN:37644 skipping SAM header line: @SQ SN:PGA_scaffold66102__1_contigs__length_38669 LN:38669 skipping SAM header line: @SQ SN:PGA_scaffold66132__1_contigs__length_39192 LN:39192 skipping SAM header line: @SQ SN:PGA_scaffold66172__1_contigs__length_62844 LN:62844 skipping SAM header line: @SQ SN:PGA_scaffold66173__1_contigs__length_34319 LN:34319 skipping SAM header line: @SQ SN:PGA_scaffold66178__1_contigs__length_49601 LN:49601 skipping SAM header line: @SQ SN:PGA_scaffold66186__1_contigs__length_45928 LN:45928 skipping SAM header line: @SQ SN:PGA_scaffold66196__1_contigs__length_53841 LN:53841 skipping SAM header line: @SQ SN:PGA_scaffold66212__1_contigs__length_32503 LN:32503 skipping SAM header line: @SQ SN:PGA_scaffold66276__1_contigs__length_39820 LN:39820 skipping SAM header line: @SQ SN:PGA_scaffold66293__1_contigs__length_33304 LN:33304 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold66641__1_contigs__length_41123 LN:41123 skipping SAM header line: @SQ SN:PGA_scaffold66642__1_contigs__length_33139 LN:33139 skipping SAM header line: @SQ SN:PGA_scaffold66656__1_contigs__length_57103 LN:57103 skipping SAM header line: @SQ SN:PGA_scaffold66666__1_contigs__length_58206 LN:58206 skipping SAM header line: @SQ SN:PGA_scaffold66674__1_contigs__length_30853 LN:30853 skipping SAM header line: @SQ SN:PGA_scaffold66682__1_contigs__length_34382 LN:34382 skipping SAM header line: @SQ SN:PGA_scaffold66713__1_contigs__length_84867 LN:84867 skipping SAM header line: @SQ SN:PGA_scaffold66717__1_contigs__length_43668 LN:43668 skipping SAM header line: @SQ SN:PGA_scaffold66721__1_contigs__length_30932 LN:30932 skipping SAM header line: @SQ SN:PGA_scaffold66729__1_contigs__length_32971 LN:32971 skipping SAM header line: @SQ SN:PGA_scaffold66737__1_contigs__length_35128 LN:35128 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold67214__1_contigs__length_49673 LN:49673 skipping SAM header line: @SQ SN:PGA_scaffold67280__1_contigs__length_31885 LN:31885 skipping SAM header line: @SQ SN:PGA_scaffold67294__1_contigs__length_50832 LN:50832 skipping SAM header line: @SQ SN:PGA_scaffold67337__1_contigs__length_52553 LN:52553 skipping SAM header line: @SQ SN:PGA_scaffold67346__1_contigs__length_32090 LN:32090 skipping SAM header line: @SQ SN:PGA_scaffold67399__1_contigs__length_33211 LN:33211 skipping SAM header line: @SQ SN:PGA_scaffold67407__1_contigs__length_73205 LN:73205 skipping SAM header line: @SQ SN:PGA_scaffold67409__1_contigs__length_30272 LN:30272 skipping SAM header line: @SQ SN:PGA_scaffold67460__1_contigs__length_63707 LN:63707 skipping SAM header line: @SQ SN:PGA_scaffold67469__1_contigs__length_34100 LN:34100 skipping SAM header line: @SQ SN:PGA_scaffold67491__1_contigs__length_36666 LN:36666 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold67633__1_contigs__length_49909 LN:49909 skipping SAM header line: @SQ SN:PGA_scaffold67641__1_contigs__length_35654 LN:35654 skipping SAM header line: @SQ SN:PGA_scaffold67666__1_contigs__length_32329 LN:32329 skipping SAM header line: @SQ SN:PGA_scaffold67687__1_contigs__length_62665 LN:62665 skipping SAM header line: @SQ SN:PGA_scaffold67702__1_contigs__length_30259 LN:30259 skipping SAM header line: @SQ SN:PGA_scaffold67709__1_contigs__length_32364 LN:32364 skipping SAM header line: @SQ SN:PGA_scaffold67745__1_contigs__length_41387 LN:41387 skipping SAM header line: @SQ SN:PGA_scaffold67754__1_contigs__length_46865 LN:46865 skipping SAM header line: @SQ SN:PGA_scaffold67781__1_contigs__length_42225 LN:42225 skipping SAM header line: @SQ SN:PGA_scaffold67782__1_contigs__length_31631 LN:31631 skipping SAM header line: @SQ SN:PGA_scaffold67794__1_contigs__length_36514 LN:36514 skipping SAM header line: @SQ SN:PGA_scaffold67874__1_contigs__length_35874 LN:35874 skipping SAM header line: @SQ SN:PGA_scaffold67908__1_contigs__length_39573 LN:39573 skipping SAM header line: @SQ SN:PGA_scaffold67909__1_contigs__length_30059 LN:30059 skipping SAM header line: @SQ SN:PGA_scaffold67921__1_contigs__length_42945 LN:42945 skipping SAM header line: @SQ SN:PGA_scaffold67942__1_contigs__length_32835 LN:32835 skipping SAM header line: @SQ SN:PGA_scaffold67967__1_contigs__length_40481 LN:40481 skipping SAM header line: @SQ SN:PGA_scaffold67981__1_contigs__length_35686 LN:35686 skipping SAM header line: @SQ SN:PGA_scaffold68035__1_contigs__length_42103 LN:42103 skipping SAM header line: @SQ SN:PGA_scaffold68039__1_contigs__length_32241 LN:32241 skipping SAM header line: @SQ SN:PGA_scaffold68042__1_contigs__length_43179 LN:43179 skipping SAM header line: @SQ SN:PGA_scaffold68044__1_contigs__length_31992 LN:31992 skipping SAM header line: @SQ SN:PGA_scaffold68049__1_contigs__length_34344 LN:34344 skipping SAM header line: @SQ SN:PGA_scaffold68053__1_contigs__length_31000 LN:31000 skipping SAM header line: @SQ SN:PGA_scaffold68092__1_contigs__length_33414 LN:33414 skipping SAM header line: @SQ SN:PGA_scaffold68150__1_contigs__length_50783 LN:50783 skipping SAM header line: @SQ SN:PGA_scaffold68157__1_contigs__length_34106 LN:34106 skipping SAM header line: @SQ SN:PGA_scaffold68184__1_contigs__length_41355 LN:41355 skipping SAM header line: @SQ SN:PGA_scaffold68193__1_contigs__length_46031 LN:46031 skipping SAM header line: @SQ SN:PGA_scaffold68205__1_contigs__length_30954 LN:30954 skipping SAM header line: @SQ SN:PGA_scaffold68252__1_contigs__length_37078 LN:37078 skipping SAM header line: @SQ SN:PGA_scaffold68273__1_contigs__length_46075 LN:46075 skipping SAM header line: @SQ SN:PGA_scaffold68274__1_contigs__length_63866 LN:63866 skipping SAM header line: @SQ SN:PGA_scaffold68332__1_contigs__length_52891 LN:52891 skipping SAM header line: @SQ SN:PGA_scaffold68355__1_contigs__length_44244 LN:44244 skipping SAM header line: @SQ SN:PGA_scaffold68382__1_contigs__length_35256 LN:35256 skipping SAM header line: @SQ SN:PGA_scaffold68387__1_contigs__length_39768 LN:39768 skipping SAM header line: @SQ SN:PGA_scaffold68394__1_contigs__length_31191 LN:31191 skipping SAM header line: @SQ SN:PGA_scaffold68395__1_contigs__length_45596 LN:45596 skipping SAM header line: @SQ SN:PGA_scaffold68397__1_contigs__length_46584 LN:46584 skipping SAM header line: @SQ SN:PGA_scaffold68425__1_contigs__length_32148 LN:32148 skipping SAM header line: @SQ SN:PGA_scaffold68455__1_contigs__length_49171 LN:49171 skipping SAM header line: @SQ SN:PGA_scaffold68463__1_contigs__length_36019 LN:36019 skipping SAM header line: @SQ SN:PGA_scaffold68476__1_contigs__length_31493 LN:31493 skipping SAM header line: @SQ SN:PGA_scaffold68483__1_contigs__length_57370 LN:57370 skipping SAM header line: @SQ SN:PGA_scaffold68484__1_contigs__length_47467 LN:47467 skipping SAM header line: @SQ SN:PGA_scaffold68495__1_contigs__length_36431 LN:36431 skipping SAM header line: @SQ SN:PGA_scaffold68498__1_contigs__length_33054 LN:33054 skipping SAM header line: @SQ SN:PGA_scaffold68529__1_contigs__length_45929 LN:45929 skipping SAM header line: @SQ SN:PGA_scaffold68536__1_contigs__length_53547 LN:53547 skipping SAM header line: @SQ SN:PGA_scaffold68576__1_contigs__length_34428 LN:34428 skipping SAM header line: @SQ SN:PGA_scaffold68613__1_contigs__length_44262 LN:44262 skipping SAM header line: @SQ SN:PGA_scaffold68674__1_contigs__length_32423 LN:32423 skipping SAM header line: @SQ SN:PGA_scaffold68721__1_contigs__length_42259 LN:42259 skipping SAM header line: @SQ SN:PGA_scaffold68747__1_contigs__length_49906 LN:49906 skipping SAM header line: @SQ SN:PGA_scaffold68765__1_contigs__length_40132 LN:40132 skipping SAM header line: @SQ SN:PGA_scaffold68777__1_contigs__length_38286 LN:38286 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold69000__1_contigs__length_31325 LN:31325 skipping SAM header line: @SQ SN:PGA_scaffold69031__1_contigs__length_49096 LN:49096 skipping SAM header line: @SQ SN:PGA_scaffold69064__1_contigs__length_30263 LN:30263 skipping SAM header line: @SQ SN:PGA_scaffold69066__1_contigs__length_41967 LN:41967 skipping SAM header line: @SQ SN:PGA_scaffold69075__1_contigs__length_43631 LN:43631 skipping SAM header line: @SQ SN:PGA_scaffold69088__1_contigs__length_46450 LN:46450 skipping SAM header line: @SQ SN:PGA_scaffold69098__1_contigs__length_35440 LN:35440 skipping SAM header line: @SQ SN:PGA_scaffold69099__1_contigs__length_56136 LN:56136 skipping SAM header line: @SQ SN:PGA_scaffold69107__1_contigs__length_36690 LN:36690 skipping SAM header line: @SQ SN:PGA_scaffold69130__1_contigs__length_52115 LN:52115 skipping SAM header line: @SQ SN:PGA_scaffold69149__1_contigs__length_34885 LN:34885 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold69439__1_contigs__length_41542 LN:41542 skipping SAM header line: @SQ SN:PGA_scaffold69451__1_contigs__length_39160 LN:39160 skipping SAM header line: @SQ SN:PGA_scaffold69459__1_contigs__length_45501 LN:45501 skipping SAM header line: @SQ SN:PGA_scaffold69477__1_contigs__length_38210 LN:38210 skipping SAM header line: @SQ SN:PGA_scaffold69479__1_contigs__length_32808 LN:32808 skipping SAM header line: @SQ SN:PGA_scaffold69484__1_contigs__length_31150 LN:31150 skipping SAM header line: @SQ SN:PGA_scaffold69495__1_contigs__length_53052 LN:53052 skipping SAM header line: @SQ SN:PGA_scaffold69529__1_contigs__length_33639 LN:33639 skipping SAM header line: @SQ SN:PGA_scaffold69555__1_contigs__length_42966 LN:42966 skipping SAM header line: @SQ SN:PGA_scaffold69556__1_contigs__length_33961 LN:33961 skipping SAM header line: @SQ SN:PGA_scaffold69619__1_contigs__length_30388 LN:30388 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold69807__1_contigs__length_45521 LN:45521 skipping SAM header line: @SQ SN:PGA_scaffold69829__1_contigs__length_34810 LN:34810 skipping SAM header line: @SQ SN:PGA_scaffold69869__1_contigs__length_52996 LN:52996 skipping SAM header line: @SQ SN:PGA_scaffold69890__1_contigs__length_34157 LN:34157 skipping SAM header line: @SQ SN:PGA_scaffold69901__1_contigs__length_33452 LN:33452 skipping SAM header line: @SQ SN:PGA_scaffold69924__1_contigs__length_44446 LN:44446 skipping SAM header line: @SQ SN:PGA_scaffold69935__1_contigs__length_32162 LN:32162 skipping SAM header line: @SQ SN:PGA_scaffold69949__1_contigs__length_34320 LN:34320 skipping SAM header line: @SQ SN:PGA_scaffold69958__1_contigs__length_74035 LN:74035 skipping SAM header line: @SQ SN:PGA_scaffold69967__1_contigs__length_33074 LN:33074 skipping SAM header line: @SQ SN:PGA_scaffold69970__1_contigs__length_36785 LN:36785 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold70204__1_contigs__length_36625 LN:36625 skipping SAM header line: @SQ SN:PGA_scaffold70218__1_contigs__length_31585 LN:31585 skipping SAM header line: @SQ SN:PGA_scaffold70228__1_contigs__length_47471 LN:47471 skipping SAM header line: @SQ SN:PGA_scaffold70241__1_contigs__length_36884 LN:36884 skipping SAM header line: @SQ SN:PGA_scaffold70287__1_contigs__length_50534 LN:50534 skipping SAM header line: @SQ SN:PGA_scaffold70301__1_contigs__length_33670 LN:33670 skipping SAM header line: @SQ SN:PGA_scaffold70359__1_contigs__length_31823 LN:31823 skipping SAM header line: @SQ SN:PGA_scaffold70409__1_contigs__length_30379 LN:30379 skipping SAM header line: @SQ SN:PGA_scaffold70448__1_contigs__length_39500 LN:39500 skipping SAM header line: @SQ SN:PGA_scaffold70468__1_contigs__length_51685 LN:51685 skipping SAM header line: @SQ SN:PGA_scaffold70474__1_contigs__length_50365 LN:50365 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold70650__1_contigs__length_36340 LN:36340 skipping SAM header line: @SQ SN:PGA_scaffold70652__1_contigs__length_42814 LN:42814 skipping SAM header line: @SQ SN:PGA_scaffold70653__1_contigs__length_42188 LN:42188 skipping SAM header line: @SQ SN:PGA_scaffold70667__1_contigs__length_31073 LN:31073 skipping SAM header line: @SQ SN:PGA_scaffold70744__1_contigs__length_41353 LN:41353 skipping SAM header line: @SQ SN:PGA_scaffold70751__1_contigs__length_35732 LN:35732 skipping SAM header line: @SQ SN:PGA_scaffold70770__1_contigs__length_37647 LN:37647 skipping SAM header line: @SQ SN:PGA_scaffold70785__1_contigs__length_35848 LN:35848 skipping SAM header line: @SQ SN:PGA_scaffold70788__1_contigs__length_34232 LN:34232 skipping SAM header line: @SQ SN:PGA_scaffold70791__1_contigs__length_36664 LN:36664 skipping SAM header line: @SQ SN:PGA_scaffold70793__1_contigs__length_31797 LN:31797 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold71023__1_contigs__length_42297 LN:42297 skipping SAM header line: @SQ SN:PGA_scaffold71024__1_contigs__length_37906 LN:37906 skipping SAM header line: @SQ SN:PGA_scaffold71080__1_contigs__length_35132 LN:35132 skipping SAM header line: @SQ SN:PGA_scaffold71087__1_contigs__length_36896 LN:36896 skipping SAM header line: @SQ SN:PGA_scaffold71091__1_contigs__length_33646 LN:33646 skipping SAM header line: @SQ SN:PGA_scaffold71150__1_contigs__length_30573 LN:30573 skipping SAM header line: @SQ SN:PGA_scaffold71155__1_contigs__length_32524 LN:32524 skipping SAM header line: @SQ SN:PGA_scaffold71157__1_contigs__length_46861 LN:46861 skipping SAM header line: @SQ SN:PGA_scaffold71196__1_contigs__length_54597 LN:54597 skipping SAM header line: @SQ SN:PGA_scaffold71206__1_contigs__length_30090 LN:30090 skipping SAM header line: @SQ SN:PGA_scaffold71207__1_contigs__length_51143 LN:51143 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold71455__1_contigs__length_57134 LN:57134 skipping SAM header line: @SQ SN:PGA_scaffold71488__1_contigs__length_33569 LN:33569 skipping SAM header line: @SQ SN:PGA_scaffold71520__1_contigs__length_33829 LN:33829 skipping SAM header line: @SQ SN:PGA_scaffold71528__1_contigs__length_39316 LN:39316 skipping SAM header line: @SQ SN:PGA_scaffold71579__1_contigs__length_45677 LN:45677 skipping SAM header line: @SQ SN:PGA_scaffold71639__1_contigs__length_32497 LN:32497 skipping SAM header line: @SQ SN:PGA_scaffold71656__1_contigs__length_30617 LN:30617 skipping SAM header line: @SQ SN:PGA_scaffold71660__1_contigs__length_43947 LN:43947 skipping SAM header line: @SQ SN:PGA_scaffold71671__1_contigs__length_34003 LN:34003 skipping SAM header line: @SQ SN:PGA_scaffold71686__1_contigs__length_54590 LN:54590 skipping SAM header line: @SQ SN:PGA_scaffold71700__1_contigs__length_34568 LN:34568 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold71876__1_contigs__length_78251 LN:78251 skipping SAM header line: @SQ SN:PGA_scaffold71891__1_contigs__length_32235 LN:32235 skipping SAM header line: @SQ SN:PGA_scaffold71897__1_contigs__length_31037 LN:31037 skipping SAM header line: @SQ SN:PGA_scaffold71912__1_contigs__length_30188 LN:30188 skipping SAM header line: @SQ SN:PGA_scaffold71942__1_contigs__length_31503 LN:31503 skipping SAM header line: @SQ SN:PGA_scaffold71943__1_contigs__length_31970 LN:31970 skipping SAM header line: @SQ SN:PGA_scaffold71993__1_contigs__length_39379 LN:39379 skipping SAM header line: @SQ SN:PGA_scaffold72048__1_contigs__length_41282 LN:41282 skipping SAM header line: @SQ SN:PGA_scaffold72054__1_contigs__length_45500 LN:45500 skipping SAM header line: @SQ SN:PGA_scaffold72055__1_contigs__length_38448 LN:38448 skipping SAM header line: @SQ SN:PGA_scaffold72064__1_contigs__length_49224 LN:49224 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold72225__1_contigs__length_34829 LN:34829 skipping SAM header line: @SQ SN:PGA_scaffold72252__1_contigs__length_37979 LN:37979 skipping SAM header line: @SQ SN:PGA_scaffold72282__1_contigs__length_53999 LN:53999 skipping SAM header line: @SQ SN:PGA_scaffold72348__1_contigs__length_34970 LN:34970 skipping SAM header line: @SQ SN:PGA_scaffold72363__1_contigs__length_51108 LN:51108 skipping SAM header line: @SQ SN:PGA_scaffold72392__1_contigs__length_64631 LN:64631 skipping SAM header line: @SQ SN:PGA_scaffold72407__1_contigs__length_43790 LN:43790 skipping SAM header line: @SQ SN:PGA_scaffold72408__1_contigs__length_33946 LN:33946 skipping SAM header line: @SQ SN:PGA_scaffold72417__1_contigs__length_30564 LN:30564 skipping SAM header line: @SQ SN:PGA_scaffold72453__1_contigs__length_31506 LN:31506 skipping SAM header line: @SQ SN:PGA_scaffold72480__1_contigs__length_68331 LN:68331 skipping SAM header line: @SQ 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LN:2213805 skipping SAM header line: @SQ SN:PGA_scaffold313646__4_contigs__length_6274882 LN:6274882 skipping SAM header line: @SQ SN:PGA_scaffold313647__2_contigs__length_5689983 LN:5689983 skipping SAM header line: @SQ SN:PGA_scaffold313648__2_contigs__length_3786466 LN:3786466 skipping SAM header line: @SQ SN:PGA_scaffold313649__2_contigs__length_2716785 LN:2716785 skipping SAM header line: @PG ID:Bismark VN:v0.19.0 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64 --samtools_path /gscratch/srlab/programs/samtools-1.9 --score_min L,0,-1.2 -I 60 -p 28 --genome /gscratch/srlab/strigg/data/Pgenr/Pgenr_070_30K/ -1 /gscratch/srlab/strigg/data/Pgenr/FASTQS/data_for_genome_comparison/EPI-113_S30_L005_R1_001_val_1.fq.gz -2 /gscratch/srlab/strigg/data/Pgenr/FASTQS/data_for_genome_comparison/EPI-113_S30_L005_R2_001_val_2.fq.gz -o /gscratch/srlab/strigg/analyses/20190214_30K" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed 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Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed 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11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Now waiting for all child processes to complete Finished processing child process. Exiting.. Merging individual splitting reports into overall report: '/gscratch/srlab/strigg/analyses/20190214_30K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: Processed 12281446 lines in total Total number of methylation call strings processed: 24562892 Final Cytosine Methylation Report ================================= Total number of C's analysed: 283891949 Total methylated C's in CpG context: 9770479 Total methylated C's in CHG context: 1412552 Total methylated C's in CHH context: 3093013 Total C to T conversions in CpG context: 28571961 Total C to T conversions in CHG context: 57535825 Total C to T conversions in CHH context: 183508119 C methylated in CpG context: 25.5% C methylated in CHG context: 2.4% C methylated in CHH context: 1.7% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 101 Maximum read length of Read 2: 99 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 101 Maximum read length of Read 2: 99 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >/gscratch/srlab/strigg/analyses/20190214_30K/< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 2G Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: Writing bedGraph to file: EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Changed directory to /gscratch/srlab/strigg/analyses/20190214_30K/ The genome of interest was specified to contain gazillions of chromosomes or scaffolds. Merging all input files and sorting everything in memory instead of writing out individual chromosome files... Writing all merged methylation calls to temp file EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz.methylation_calls.merged Finished writing methylation calls from CpG_OT_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt to merged temp file Finished writing methylation calls from CpG_OB_EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt to merged temp file Sorting input file EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz.methylation_calls.merged by positions (using -S of 2G) Successfully deleted the temporary input file EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz.methylation_calls.merged Finished BedGraph conversion ... Checking file >>/gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... ...passed! Writing result file containing methylation information for C in CpG context from the original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:PGA_scaffold1__77_contigs__length_89643857 LN:89643857 skipping SAM header line: @SQ SN:PGA_scaffold2__36_contigs__length_69596280 LN:69596280 skipping SAM header line: @SQ SN:PGA_scaffold3__111_contigs__length_57743597 LN:57743597 skipping SAM header line: @SQ SN:PGA_scaffold4__129_contigs__length_65288255 LN:65288255 skipping SAM header line: @SQ SN:PGA_scaffold5__109_contigs__length_67248332 LN:67248332 skipping SAM header line: @SQ SN:PGA_scaffold6__104_contigs__length_61759565 LN:61759565 skipping SAM header line: @SQ SN:PGA_scaffold7__69_contigs__length_43120122 LN:43120122 skipping SAM header line: @SQ SN:PGA_scaffold8__63_contigs__length_61151155 LN:61151155 skipping SAM header line: @SQ SN:PGA_scaffold9__45_contigs__length_38581958 LN:38581958 skipping SAM header line: @SQ SN:PGA_scaffold10__49_contigs__length_53961475 LN:53961475 skipping SAM header line: @SQ SN:PGA_scaffold11__79_contigs__length_51449921 LN:51449921 skipping SAM header line: @SQ SN:PGA_scaffold12__71_contigs__length_50438331 LN:50438331 skipping SAM header line: @SQ SN:PGA_scaffold13__52_contigs__length_44396874 LN:44396874 skipping SAM header line: @SQ SN:PGA_scaffold14__91_contigs__length_45393038 LN:45393038 skipping SAM header line: @SQ SN:PGA_scaffold15__101_contigs__length_47938513 LN:47938513 skipping SAM header line: @SQ SN:PGA_scaffold16__33_contigs__length_31980953 LN:31980953 skipping SAM header line: @SQ SN:PGA_scaffold17__51_contigs__length_34923512 LN:34923512 skipping SAM header line: @SQ SN:PGA_scaffold18__69_contigs__length_27737463 LN:27737463 skipping SAM header line: @SQ SN:PGA_scaffold100__1_contigs__length_37850 LN:37850 skipping SAM header line: @SQ SN:PGA_scaffold101__1_contigs__length_30584 LN:30584 skipping SAM header line: @SQ SN:PGA_scaffold102__1_contigs__length_45787 LN:45787 skipping SAM header line: @SQ SN:PGA_scaffold104__1_contigs__length_44353 LN:44353 skipping SAM header line: @SQ SN:PGA_scaffold105__1_contigs__length_50812 LN:50812 skipping SAM header line: @SQ SN:PGA_scaffold108__1_contigs__length_37373 LN:37373 skipping SAM header line: @SQ SN:PGA_scaffold109__1_contigs__length_31266 LN:31266 skipping SAM header line: @SQ SN:PGA_scaffold117__1_contigs__length_33965 LN:33965 skipping SAM header line: @SQ SN:PGA_scaffold118__1_contigs__length_78741 LN:78741 skipping SAM header line: @SQ SN:PGA_scaffold120__1_contigs__length_36635 LN:36635 skipping SAM header line: @SQ SN:PGA_scaffold122__1_contigs__length_44468 LN:44468 skipping SAM header line: @SQ SN:PGA_scaffold123__1_contigs__length_30311 LN:30311 skipping SAM header line: @SQ SN:PGA_scaffold124__1_contigs__length_35932 LN:35932 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold52892__1_contigs__length_39173 LN:39173 skipping SAM header line: @SQ SN:PGA_scaffold52893__1_contigs__length_36559 LN:36559 skipping SAM header line: @SQ SN:PGA_scaffold52914__1_contigs__length_54588 LN:54588 skipping SAM header line: @SQ SN:PGA_scaffold52916__1_contigs__length_30509 LN:30509 skipping SAM header line: @SQ SN:PGA_scaffold52973__1_contigs__length_33821 LN:33821 skipping SAM header line: @SQ SN:PGA_scaffold52979__1_contigs__length_30521 LN:30521 skipping SAM header line: @SQ SN:PGA_scaffold53021__1_contigs__length_37828 LN:37828 skipping SAM header line: @SQ SN:PGA_scaffold53196__1_contigs__length_30707 LN:30707 skipping SAM header line: @SQ SN:PGA_scaffold53343__1_contigs__length_31364 LN:31364 skipping SAM header line: @SQ SN:PGA_scaffold53591__1_contigs__length_57201 LN:57201 skipping SAM header line: @SQ SN:PGA_scaffold53633__1_contigs__length_37396 LN:37396 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold53990__1_contigs__length_50335 LN:50335 skipping SAM header line: @SQ SN:PGA_scaffold53991__1_contigs__length_51016 LN:51016 skipping SAM header line: @SQ SN:PGA_scaffold53992__1_contigs__length_30819 LN:30819 skipping SAM header line: @SQ SN:PGA_scaffold53994__1_contigs__length_45288 LN:45288 skipping SAM header line: @SQ SN:PGA_scaffold53995__1_contigs__length_36326 LN:36326 skipping SAM header line: @SQ SN:PGA_scaffold54015__1_contigs__length_30408 LN:30408 skipping SAM header line: @SQ SN:PGA_scaffold54205__1_contigs__length_38797 LN:38797 skipping SAM header line: @SQ SN:PGA_scaffold54342__1_contigs__length_60814 LN:60814 skipping SAM header line: @SQ SN:PGA_scaffold54901__1_contigs__length_37309 LN:37309 skipping SAM header line: @SQ SN:PGA_scaffold54965__1_contigs__length_38374 LN:38374 skipping SAM header line: @SQ SN:PGA_scaffold54999__1_contigs__length_31291 LN:31291 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold55378__1_contigs__length_34882 LN:34882 skipping SAM header line: @SQ SN:PGA_scaffold55381__1_contigs__length_30600 LN:30600 skipping SAM header line: @SQ SN:PGA_scaffold55711__1_contigs__length_39787 LN:39787 skipping SAM header line: @SQ SN:PGA_scaffold55714__1_contigs__length_36372 LN:36372 skipping SAM header line: @SQ SN:PGA_scaffold55745__1_contigs__length_39718 LN:39718 skipping SAM header line: @SQ SN:PGA_scaffold55747__1_contigs__length_42692 LN:42692 skipping SAM header line: @SQ SN:PGA_scaffold55855__1_contigs__length_34489 LN:34489 skipping SAM header line: @SQ SN:PGA_scaffold55860__1_contigs__length_50662 LN:50662 skipping SAM header line: @SQ SN:PGA_scaffold55979__1_contigs__length_33616 LN:33616 skipping SAM header line: @SQ SN:PGA_scaffold56091__1_contigs__length_39544 LN:39544 skipping SAM header line: @SQ SN:PGA_scaffold56365__1_contigs__length_56315 LN:56315 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold57070__1_contigs__length_35859 LN:35859 skipping SAM header line: @SQ SN:PGA_scaffold57215__1_contigs__length_43048 LN:43048 skipping SAM header line: @SQ SN:PGA_scaffold57298__1_contigs__length_31957 LN:31957 skipping SAM header line: @SQ SN:PGA_scaffold57301__1_contigs__length_34452 LN:34452 skipping SAM header line: @SQ SN:PGA_scaffold57398__1_contigs__length_30770 LN:30770 skipping SAM header line: @SQ SN:PGA_scaffold57419__1_contigs__length_56657 LN:56657 skipping SAM header line: @SQ SN:PGA_scaffold57435__1_contigs__length_39348 LN:39348 skipping SAM header line: @SQ SN:PGA_scaffold57479__1_contigs__length_34907 LN:34907 skipping SAM header line: @SQ SN:PGA_scaffold57490__1_contigs__length_31663 LN:31663 skipping SAM header line: @SQ SN:PGA_scaffold57492__1_contigs__length_41689 LN:41689 skipping SAM header line: @SQ SN:PGA_scaffold57510__1_contigs__length_39178 LN:39178 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold57901__1_contigs__length_33405 LN:33405 skipping SAM header line: @SQ SN:PGA_scaffold58076__1_contigs__length_32943 LN:32943 skipping SAM header line: @SQ SN:PGA_scaffold58238__1_contigs__length_68373 LN:68373 skipping SAM header line: @SQ SN:PGA_scaffold58264__1_contigs__length_33661 LN:33661 skipping SAM header line: @SQ SN:PGA_scaffold58284__1_contigs__length_33826 LN:33826 skipping SAM header line: @SQ SN:PGA_scaffold58361__1_contigs__length_52751 LN:52751 skipping SAM header line: @SQ SN:PGA_scaffold58366__1_contigs__length_37326 LN:37326 skipping SAM header line: @SQ SN:PGA_scaffold58382__1_contigs__length_41713 LN:41713 skipping SAM header line: @SQ SN:PGA_scaffold58383__1_contigs__length_44366 LN:44366 skipping SAM header line: @SQ SN:PGA_scaffold58388__1_contigs__length_32850 LN:32850 skipping SAM header line: @SQ SN:PGA_scaffold58393__1_contigs__length_33434 LN:33434 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold58676__1_contigs__length_42740 LN:42740 skipping SAM header line: @SQ SN:PGA_scaffold58700__1_contigs__length_38161 LN:38161 skipping SAM header line: @SQ SN:PGA_scaffold58712__1_contigs__length_43972 LN:43972 skipping SAM header line: @SQ SN:PGA_scaffold58749__1_contigs__length_42553 LN:42553 skipping SAM header line: @SQ SN:PGA_scaffold58976__1_contigs__length_32077 LN:32077 skipping SAM header line: @SQ SN:PGA_scaffold58984__1_contigs__length_30766 LN:30766 skipping SAM header line: @SQ SN:PGA_scaffold59041__1_contigs__length_49211 LN:49211 skipping SAM header line: @SQ SN:PGA_scaffold59054__1_contigs__length_30586 LN:30586 skipping SAM header line: @SQ SN:PGA_scaffold59056__1_contigs__length_33360 LN:33360 skipping SAM header line: @SQ SN:PGA_scaffold59166__1_contigs__length_30875 LN:30875 skipping SAM header line: @SQ SN:PGA_scaffold59171__1_contigs__length_43122 LN:43122 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold59687__1_contigs__length_34469 LN:34469 skipping SAM header line: @SQ SN:PGA_scaffold59721__1_contigs__length_42511 LN:42511 skipping SAM header line: @SQ SN:PGA_scaffold59723__1_contigs__length_39806 LN:39806 skipping SAM header line: @SQ SN:PGA_scaffold59727__1_contigs__length_31211 LN:31211 skipping SAM header line: @SQ SN:PGA_scaffold59729__1_contigs__length_32301 LN:32301 skipping SAM header line: @SQ SN:PGA_scaffold59750__1_contigs__length_36894 LN:36894 skipping SAM header line: @SQ SN:PGA_scaffold59858__1_contigs__length_39712 LN:39712 skipping SAM header line: @SQ SN:PGA_scaffold59872__1_contigs__length_51346 LN:51346 skipping SAM header line: @SQ SN:PGA_scaffold59907__1_contigs__length_30865 LN:30865 skipping SAM header line: @SQ SN:PGA_scaffold59933__1_contigs__length_43116 LN:43116 skipping SAM header line: @SQ SN:PGA_scaffold59943__1_contigs__length_33685 LN:33685 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold60318__1_contigs__length_36247 LN:36247 skipping SAM header line: @SQ SN:PGA_scaffold60412__1_contigs__length_79597 LN:79597 skipping SAM header line: @SQ SN:PGA_scaffold60413__1_contigs__length_42267 LN:42267 skipping SAM header line: @SQ SN:PGA_scaffold60414__1_contigs__length_49107 LN:49107 skipping SAM header line: @SQ SN:PGA_scaffold60441__1_contigs__length_43835 LN:43835 skipping SAM header line: @SQ SN:PGA_scaffold60488__1_contigs__length_50761 LN:50761 skipping SAM header line: @SQ SN:PGA_scaffold60490__1_contigs__length_43435 LN:43435 skipping SAM header line: @SQ SN:PGA_scaffold60615__1_contigs__length_40286 LN:40286 skipping SAM header line: @SQ SN:PGA_scaffold60652__1_contigs__length_52273 LN:52273 skipping SAM header line: @SQ SN:PGA_scaffold60657__1_contigs__length_45209 LN:45209 skipping SAM header line: @SQ SN:PGA_scaffold60699__1_contigs__length_33841 LN:33841 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold61037__1_contigs__length_31360 LN:31360 skipping SAM header line: @SQ SN:PGA_scaffold61040__1_contigs__length_32690 LN:32690 skipping SAM header line: @SQ SN:PGA_scaffold61046__1_contigs__length_41502 LN:41502 skipping SAM header line: @SQ SN:PGA_scaffold61063__1_contigs__length_47605 LN:47605 skipping SAM header line: @SQ SN:PGA_scaffold61072__1_contigs__length_33361 LN:33361 skipping SAM header line: @SQ SN:PGA_scaffold61096__1_contigs__length_30606 LN:30606 skipping SAM header line: @SQ SN:PGA_scaffold61099__1_contigs__length_49058 LN:49058 skipping SAM header line: @SQ SN:PGA_scaffold61103__1_contigs__length_37635 LN:37635 skipping SAM header line: @SQ SN:PGA_scaffold61168__1_contigs__length_30806 LN:30806 skipping SAM header line: @SQ SN:PGA_scaffold61190__1_contigs__length_38143 LN:38143 skipping SAM header line: @SQ SN:PGA_scaffold61242__1_contigs__length_43427 LN:43427 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold61392__1_contigs__length_57213 LN:57213 skipping SAM header line: @SQ SN:PGA_scaffold61445__1_contigs__length_33748 LN:33748 skipping SAM header line: @SQ SN:PGA_scaffold61589__1_contigs__length_43181 LN:43181 skipping SAM header line: @SQ SN:PGA_scaffold61592__1_contigs__length_35695 LN:35695 skipping SAM header line: @SQ SN:PGA_scaffold61615__1_contigs__length_40934 LN:40934 skipping SAM header line: @SQ SN:PGA_scaffold61619__1_contigs__length_33825 LN:33825 skipping SAM header line: @SQ SN:PGA_scaffold61628__1_contigs__length_48494 LN:48494 skipping SAM header line: @SQ SN:PGA_scaffold61636__1_contigs__length_47931 LN:47931 skipping SAM header line: @SQ SN:PGA_scaffold61640__1_contigs__length_57604 LN:57604 skipping SAM header line: @SQ SN:PGA_scaffold61646__1_contigs__length_55250 LN:55250 skipping SAM header line: @SQ SN:PGA_scaffold61650__1_contigs__length_31696 LN:31696 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold61897__1_contigs__length_41371 LN:41371 skipping SAM header line: @SQ SN:PGA_scaffold61982__1_contigs__length_37118 LN:37118 skipping SAM header line: @SQ SN:PGA_scaffold62098__1_contigs__length_77920 LN:77920 skipping SAM header line: @SQ SN:PGA_scaffold62138__1_contigs__length_33334 LN:33334 skipping SAM header line: @SQ SN:PGA_scaffold62168__1_contigs__length_43227 LN:43227 skipping SAM header line: @SQ SN:PGA_scaffold62186__1_contigs__length_43446 LN:43446 skipping SAM header line: @SQ SN:PGA_scaffold62192__1_contigs__length_56278 LN:56278 skipping SAM header line: @SQ SN:PGA_scaffold62198__1_contigs__length_38255 LN:38255 skipping SAM header line: @SQ SN:PGA_scaffold62206__1_contigs__length_67303 LN:67303 skipping SAM header line: @SQ SN:PGA_scaffold62224__1_contigs__length_74638 LN:74638 skipping SAM header line: @SQ SN:PGA_scaffold62226__1_contigs__length_86027 LN:86027 skipping SAM header line: @SQ SN:PGA_scaffold62319__1_contigs__length_36955 LN:36955 skipping SAM header line: @SQ SN:PGA_scaffold62364__1_contigs__length_54322 LN:54322 skipping SAM header line: @SQ SN:PGA_scaffold62403__1_contigs__length_38564 LN:38564 skipping SAM header line: @SQ SN:PGA_scaffold62435__1_contigs__length_59037 LN:59037 skipping SAM header line: @SQ SN:PGA_scaffold62443__1_contigs__length_39499 LN:39499 skipping SAM header line: @SQ SN:PGA_scaffold62455__1_contigs__length_63754 LN:63754 skipping SAM header line: @SQ SN:PGA_scaffold62459__1_contigs__length_42719 LN:42719 skipping SAM header line: @SQ SN:PGA_scaffold62475__1_contigs__length_44394 LN:44394 skipping SAM header line: @SQ SN:PGA_scaffold62477__1_contigs__length_40507 LN:40507 skipping SAM header line: @SQ SN:PGA_scaffold62478__1_contigs__length_48273 LN:48273 skipping SAM header line: @SQ SN:PGA_scaffold62492__1_contigs__length_31702 LN:31702 skipping SAM header line: @SQ SN:PGA_scaffold62537__1_contigs__length_39343 LN:39343 skipping SAM header line: @SQ SN:PGA_scaffold62542__1_contigs__length_32921 LN:32921 skipping SAM header line: @SQ SN:PGA_scaffold62544__1_contigs__length_41829 LN:41829 skipping SAM header line: @SQ SN:PGA_scaffold62591__1_contigs__length_64464 LN:64464 skipping SAM header line: @SQ SN:PGA_scaffold62601__1_contigs__length_36053 LN:36053 skipping SAM header line: @SQ SN:PGA_scaffold62602__1_contigs__length_30153 LN:30153 skipping SAM header line: @SQ SN:PGA_scaffold62661__1_contigs__length_34648 LN:34648 skipping SAM header line: @SQ SN:PGA_scaffold62687__1_contigs__length_56019 LN:56019 skipping SAM header line: @SQ SN:PGA_scaffold62752__1_contigs__length_33202 LN:33202 skipping SAM header line: @SQ SN:PGA_scaffold62854__1_contigs__length_47148 LN:47148 skipping SAM header line: @SQ SN:PGA_scaffold62862__1_contigs__length_31728 LN:31728 skipping SAM header line: @SQ SN:PGA_scaffold62865__1_contigs__length_39731 LN:39731 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold62982__1_contigs__length_51892 LN:51892 skipping SAM header line: @SQ SN:PGA_scaffold63008__1_contigs__length_40245 LN:40245 skipping SAM header line: @SQ SN:PGA_scaffold63022__1_contigs__length_31832 LN:31832 skipping SAM header line: @SQ SN:PGA_scaffold63033__1_contigs__length_64137 LN:64137 skipping SAM header line: @SQ SN:PGA_scaffold63057__1_contigs__length_30046 LN:30046 skipping SAM header line: @SQ SN:PGA_scaffold63059__1_contigs__length_30993 LN:30993 skipping SAM header line: @SQ SN:PGA_scaffold63073__1_contigs__length_59843 LN:59843 skipping SAM header line: @SQ SN:PGA_scaffold63076__1_contigs__length_65566 LN:65566 skipping SAM header line: @SQ SN:PGA_scaffold63085__1_contigs__length_46291 LN:46291 skipping SAM header line: @SQ SN:PGA_scaffold63090__1_contigs__length_43963 LN:43963 skipping SAM header line: @SQ SN:PGA_scaffold63101__1_contigs__length_45043 LN:45043 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold63286__1_contigs__length_64799 LN:64799 skipping SAM header line: @SQ SN:PGA_scaffold63295__1_contigs__length_73242 LN:73242 skipping SAM header line: @SQ SN:PGA_scaffold63327__1_contigs__length_41823 LN:41823 skipping SAM header line: @SQ SN:PGA_scaffold63330__1_contigs__length_32542 LN:32542 skipping SAM header line: @SQ SN:PGA_scaffold63338__1_contigs__length_30825 LN:30825 skipping SAM header line: @SQ SN:PGA_scaffold63480__1_contigs__length_39812 LN:39812 skipping SAM header line: @SQ SN:PGA_scaffold63504__1_contigs__length_83511 LN:83511 skipping SAM header line: @SQ SN:PGA_scaffold63543__1_contigs__length_67322 LN:67322 skipping SAM header line: @SQ SN:PGA_scaffold63594__1_contigs__length_42878 LN:42878 skipping SAM header line: @SQ SN:PGA_scaffold63635__1_contigs__length_31389 LN:31389 skipping SAM header line: @SQ SN:PGA_scaffold63681__1_contigs__length_35508 LN:35508 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold63883__1_contigs__length_68258 LN:68258 skipping SAM header line: @SQ SN:PGA_scaffold63886__1_contigs__length_33964 LN:33964 skipping SAM header line: @SQ SN:PGA_scaffold63901__1_contigs__length_33737 LN:33737 skipping SAM header line: @SQ SN:PGA_scaffold63902__1_contigs__length_30981 LN:30981 skipping SAM header line: @SQ SN:PGA_scaffold63904__1_contigs__length_56940 LN:56940 skipping SAM header line: @SQ SN:PGA_scaffold63956__1_contigs__length_32393 LN:32393 skipping SAM header line: @SQ SN:PGA_scaffold63976__1_contigs__length_45677 LN:45677 skipping SAM header line: @SQ SN:PGA_scaffold63999__1_contigs__length_42041 LN:42041 skipping SAM header line: @SQ SN:PGA_scaffold64025__1_contigs__length_40125 LN:40125 skipping SAM header line: @SQ SN:PGA_scaffold64049__1_contigs__length_45875 LN:45875 skipping SAM header line: @SQ SN:PGA_scaffold64054__1_contigs__length_70679 LN:70679 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold64383__1_contigs__length_88001 LN:88001 skipping SAM header line: @SQ SN:PGA_scaffold64394__1_contigs__length_33404 LN:33404 skipping SAM header line: @SQ SN:PGA_scaffold64426__1_contigs__length_49077 LN:49077 skipping SAM header line: @SQ SN:PGA_scaffold64456__1_contigs__length_38607 LN:38607 skipping SAM header line: @SQ SN:PGA_scaffold64462__1_contigs__length_30068 LN:30068 skipping SAM header line: @SQ SN:PGA_scaffold64465__1_contigs__length_42085 LN:42085 skipping SAM header line: @SQ SN:PGA_scaffold64466__1_contigs__length_38330 LN:38330 skipping SAM header line: @SQ SN:PGA_scaffold64470__1_contigs__length_48847 LN:48847 skipping SAM header line: @SQ SN:PGA_scaffold64471__1_contigs__length_34271 LN:34271 skipping SAM header line: @SQ SN:PGA_scaffold64473__1_contigs__length_33437 LN:33437 skipping SAM header line: @SQ SN:PGA_scaffold64529__1_contigs__length_33564 LN:33564 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold65693__1_contigs__length_34648 LN:34648 skipping SAM header line: @SQ SN:PGA_scaffold65737__1_contigs__length_37231 LN:37231 skipping SAM header line: @SQ SN:PGA_scaffold65765__1_contigs__length_39217 LN:39217 skipping SAM header line: @SQ SN:PGA_scaffold65777__1_contigs__length_32724 LN:32724 skipping SAM header line: @SQ SN:PGA_scaffold65781__1_contigs__length_42302 LN:42302 skipping SAM header line: @SQ SN:PGA_scaffold65786__1_contigs__length_56597 LN:56597 skipping SAM header line: @SQ SN:PGA_scaffold65807__1_contigs__length_35379 LN:35379 skipping SAM header line: @SQ SN:PGA_scaffold65809__1_contigs__length_36632 LN:36632 skipping SAM header line: @SQ SN:PGA_scaffold65815__1_contigs__length_49308 LN:49308 skipping SAM header line: @SQ SN:PGA_scaffold65846__1_contigs__length_33986 LN:33986 skipping SAM header line: @SQ SN:PGA_scaffold65851__1_contigs__length_44621 LN:44621 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold66079__1_contigs__length_37644 LN:37644 skipping SAM header line: @SQ SN:PGA_scaffold66102__1_contigs__length_38669 LN:38669 skipping SAM header line: @SQ SN:PGA_scaffold66132__1_contigs__length_39192 LN:39192 skipping SAM header line: @SQ SN:PGA_scaffold66172__1_contigs__length_62844 LN:62844 skipping SAM header line: @SQ SN:PGA_scaffold66173__1_contigs__length_34319 LN:34319 skipping SAM header line: @SQ SN:PGA_scaffold66178__1_contigs__length_49601 LN:49601 skipping SAM header line: @SQ SN:PGA_scaffold66186__1_contigs__length_45928 LN:45928 skipping SAM header line: @SQ SN:PGA_scaffold66196__1_contigs__length_53841 LN:53841 skipping SAM header line: @SQ SN:PGA_scaffold66212__1_contigs__length_32503 LN:32503 skipping SAM header line: @SQ SN:PGA_scaffold66276__1_contigs__length_39820 LN:39820 skipping SAM header line: @SQ SN:PGA_scaffold66293__1_contigs__length_33304 LN:33304 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold66641__1_contigs__length_41123 LN:41123 skipping SAM header line: @SQ SN:PGA_scaffold66642__1_contigs__length_33139 LN:33139 skipping SAM header line: @SQ SN:PGA_scaffold66656__1_contigs__length_57103 LN:57103 skipping SAM header line: @SQ SN:PGA_scaffold66666__1_contigs__length_58206 LN:58206 skipping SAM header line: @SQ SN:PGA_scaffold66674__1_contigs__length_30853 LN:30853 skipping SAM header line: @SQ SN:PGA_scaffold66682__1_contigs__length_34382 LN:34382 skipping SAM header line: @SQ SN:PGA_scaffold66713__1_contigs__length_84867 LN:84867 skipping SAM header line: @SQ SN:PGA_scaffold66717__1_contigs__length_43668 LN:43668 skipping SAM header line: @SQ SN:PGA_scaffold66721__1_contigs__length_30932 LN:30932 skipping SAM header line: @SQ SN:PGA_scaffold66729__1_contigs__length_32971 LN:32971 skipping SAM header line: @SQ SN:PGA_scaffold66737__1_contigs__length_35128 LN:35128 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold67214__1_contigs__length_49673 LN:49673 skipping SAM header line: @SQ SN:PGA_scaffold67280__1_contigs__length_31885 LN:31885 skipping SAM header line: @SQ SN:PGA_scaffold67294__1_contigs__length_50832 LN:50832 skipping SAM header line: @SQ SN:PGA_scaffold67337__1_contigs__length_52553 LN:52553 skipping SAM header line: @SQ SN:PGA_scaffold67346__1_contigs__length_32090 LN:32090 skipping SAM header line: @SQ SN:PGA_scaffold67399__1_contigs__length_33211 LN:33211 skipping SAM header line: @SQ SN:PGA_scaffold67407__1_contigs__length_73205 LN:73205 skipping SAM header line: @SQ SN:PGA_scaffold67409__1_contigs__length_30272 LN:30272 skipping SAM header line: @SQ SN:PGA_scaffold67460__1_contigs__length_63707 LN:63707 skipping SAM header line: @SQ SN:PGA_scaffold67469__1_contigs__length_34100 LN:34100 skipping SAM header line: @SQ SN:PGA_scaffold67491__1_contigs__length_36666 LN:36666 skipping SAM header line: @SQ SN:PGA_scaffold67492__1_contigs__length_32377 LN:32377 skipping SAM header line: @SQ SN:PGA_scaffold67500__1_contigs__length_32321 LN:32321 skipping SAM header line: @SQ SN:PGA_scaffold67501__1_contigs__length_45282 LN:45282 skipping SAM header line: @SQ SN:PGA_scaffold67506__1_contigs__length_34916 LN:34916 skipping SAM header line: @SQ SN:PGA_scaffold67532__1_contigs__length_45886 LN:45886 skipping SAM header line: @SQ SN:PGA_scaffold67555__1_contigs__length_42738 LN:42738 skipping SAM header line: @SQ SN:PGA_scaffold67566__1_contigs__length_131359 LN:131359 skipping SAM header line: @SQ SN:PGA_scaffold67576__1_contigs__length_71513 LN:71513 skipping SAM header line: @SQ SN:PGA_scaffold67579__1_contigs__length_30606 LN:30606 skipping SAM header line: @SQ SN:PGA_scaffold67604__1_contigs__length_30533 LN:30533 skipping SAM header line: @SQ SN:PGA_scaffold67614__1_contigs__length_45881 LN:45881 skipping SAM header line: @SQ SN:PGA_scaffold67629__1_contigs__length_41418 LN:41418 skipping SAM header line: @SQ SN:PGA_scaffold67633__1_contigs__length_49909 LN:49909 skipping SAM header line: @SQ SN:PGA_scaffold67641__1_contigs__length_35654 LN:35654 skipping SAM header line: @SQ SN:PGA_scaffold67666__1_contigs__length_32329 LN:32329 skipping SAM header line: @SQ SN:PGA_scaffold67687__1_contigs__length_62665 LN:62665 skipping SAM header line: @SQ SN:PGA_scaffold67702__1_contigs__length_30259 LN:30259 skipping SAM header line: @SQ SN:PGA_scaffold67709__1_contigs__length_32364 LN:32364 skipping SAM header line: @SQ SN:PGA_scaffold67745__1_contigs__length_41387 LN:41387 skipping SAM header line: @SQ SN:PGA_scaffold67754__1_contigs__length_46865 LN:46865 skipping SAM header line: @SQ SN:PGA_scaffold67781__1_contigs__length_42225 LN:42225 skipping SAM header line: @SQ SN:PGA_scaffold67782__1_contigs__length_31631 LN:31631 skipping SAM header line: @SQ SN:PGA_scaffold67794__1_contigs__length_36514 LN:36514 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold68053__1_contigs__length_31000 LN:31000 skipping SAM header line: @SQ SN:PGA_scaffold68092__1_contigs__length_33414 LN:33414 skipping SAM header line: @SQ SN:PGA_scaffold68150__1_contigs__length_50783 LN:50783 skipping SAM header line: @SQ SN:PGA_scaffold68157__1_contigs__length_34106 LN:34106 skipping SAM header line: @SQ SN:PGA_scaffold68184__1_contigs__length_41355 LN:41355 skipping SAM header line: @SQ SN:PGA_scaffold68193__1_contigs__length_46031 LN:46031 skipping SAM header line: @SQ SN:PGA_scaffold68205__1_contigs__length_30954 LN:30954 skipping SAM header line: @SQ SN:PGA_scaffold68252__1_contigs__length_37078 LN:37078 skipping SAM header line: @SQ SN:PGA_scaffold68273__1_contigs__length_46075 LN:46075 skipping SAM header line: @SQ SN:PGA_scaffold68274__1_contigs__length_63866 LN:63866 skipping SAM header line: @SQ SN:PGA_scaffold68332__1_contigs__length_52891 LN:52891 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold68495__1_contigs__length_36431 LN:36431 skipping SAM header line: @SQ SN:PGA_scaffold68498__1_contigs__length_33054 LN:33054 skipping SAM header line: @SQ SN:PGA_scaffold68529__1_contigs__length_45929 LN:45929 skipping SAM header line: @SQ SN:PGA_scaffold68536__1_contigs__length_53547 LN:53547 skipping SAM header line: @SQ SN:PGA_scaffold68576__1_contigs__length_34428 LN:34428 skipping SAM header line: @SQ SN:PGA_scaffold68613__1_contigs__length_44262 LN:44262 skipping SAM header line: @SQ SN:PGA_scaffold68674__1_contigs__length_32423 LN:32423 skipping SAM header line: @SQ SN:PGA_scaffold68721__1_contigs__length_42259 LN:42259 skipping SAM header line: @SQ SN:PGA_scaffold68747__1_contigs__length_49906 LN:49906 skipping SAM header line: @SQ SN:PGA_scaffold68765__1_contigs__length_40132 LN:40132 skipping SAM header line: @SQ SN:PGA_scaffold68777__1_contigs__length_38286 LN:38286 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold69000__1_contigs__length_31325 LN:31325 skipping SAM header line: @SQ SN:PGA_scaffold69031__1_contigs__length_49096 LN:49096 skipping SAM header line: @SQ SN:PGA_scaffold69064__1_contigs__length_30263 LN:30263 skipping SAM header line: @SQ SN:PGA_scaffold69066__1_contigs__length_41967 LN:41967 skipping SAM header line: @SQ SN:PGA_scaffold69075__1_contigs__length_43631 LN:43631 skipping SAM header line: @SQ SN:PGA_scaffold69088__1_contigs__length_46450 LN:46450 skipping SAM header line: @SQ SN:PGA_scaffold69098__1_contigs__length_35440 LN:35440 skipping SAM header line: @SQ SN:PGA_scaffold69099__1_contigs__length_56136 LN:56136 skipping SAM header line: @SQ SN:PGA_scaffold69107__1_contigs__length_36690 LN:36690 skipping SAM header line: @SQ SN:PGA_scaffold69130__1_contigs__length_52115 LN:52115 skipping SAM header line: @SQ SN:PGA_scaffold69149__1_contigs__length_34885 LN:34885 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold69807__1_contigs__length_45521 LN:45521 skipping SAM header line: @SQ SN:PGA_scaffold69829__1_contigs__length_34810 LN:34810 skipping SAM header line: @SQ SN:PGA_scaffold69869__1_contigs__length_52996 LN:52996 skipping SAM header line: @SQ SN:PGA_scaffold69890__1_contigs__length_34157 LN:34157 skipping SAM header line: @SQ SN:PGA_scaffold69901__1_contigs__length_33452 LN:33452 skipping SAM header line: @SQ SN:PGA_scaffold69924__1_contigs__length_44446 LN:44446 skipping SAM header line: @SQ SN:PGA_scaffold69935__1_contigs__length_32162 LN:32162 skipping SAM header line: @SQ SN:PGA_scaffold69949__1_contigs__length_34320 LN:34320 skipping SAM header line: @SQ SN:PGA_scaffold69958__1_contigs__length_74035 LN:74035 skipping SAM header line: @SQ SN:PGA_scaffold69967__1_contigs__length_33074 LN:33074 skipping SAM header line: @SQ SN:PGA_scaffold69970__1_contigs__length_36785 LN:36785 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold70650__1_contigs__length_36340 LN:36340 skipping SAM header line: @SQ SN:PGA_scaffold70652__1_contigs__length_42814 LN:42814 skipping SAM header line: @SQ SN:PGA_scaffold70653__1_contigs__length_42188 LN:42188 skipping SAM header line: @SQ SN:PGA_scaffold70667__1_contigs__length_31073 LN:31073 skipping SAM header line: @SQ SN:PGA_scaffold70744__1_contigs__length_41353 LN:41353 skipping SAM header line: @SQ SN:PGA_scaffold70751__1_contigs__length_35732 LN:35732 skipping SAM header line: @SQ SN:PGA_scaffold70770__1_contigs__length_37647 LN:37647 skipping SAM header line: @SQ SN:PGA_scaffold70785__1_contigs__length_35848 LN:35848 skipping SAM header line: @SQ SN:PGA_scaffold70788__1_contigs__length_34232 LN:34232 skipping SAM header line: @SQ SN:PGA_scaffold70791__1_contigs__length_36664 LN:36664 skipping SAM header line: @SQ SN:PGA_scaffold70793__1_contigs__length_31797 LN:31797 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold71023__1_contigs__length_42297 LN:42297 skipping SAM header line: @SQ SN:PGA_scaffold71024__1_contigs__length_37906 LN:37906 skipping SAM header line: @SQ SN:PGA_scaffold71080__1_contigs__length_35132 LN:35132 skipping SAM header line: @SQ SN:PGA_scaffold71087__1_contigs__length_36896 LN:36896 skipping SAM header line: @SQ SN:PGA_scaffold71091__1_contigs__length_33646 LN:33646 skipping SAM header line: @SQ SN:PGA_scaffold71150__1_contigs__length_30573 LN:30573 skipping SAM header line: @SQ SN:PGA_scaffold71155__1_contigs__length_32524 LN:32524 skipping SAM header line: @SQ SN:PGA_scaffold71157__1_contigs__length_46861 LN:46861 skipping SAM header line: @SQ SN:PGA_scaffold71196__1_contigs__length_54597 LN:54597 skipping SAM header line: @SQ SN:PGA_scaffold71206__1_contigs__length_30090 LN:30090 skipping SAM header line: @SQ SN:PGA_scaffold71207__1_contigs__length_51143 LN:51143 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold71455__1_contigs__length_57134 LN:57134 skipping SAM header line: @SQ SN:PGA_scaffold71488__1_contigs__length_33569 LN:33569 skipping SAM header line: @SQ SN:PGA_scaffold71520__1_contigs__length_33829 LN:33829 skipping SAM header line: @SQ SN:PGA_scaffold71528__1_contigs__length_39316 LN:39316 skipping SAM header line: @SQ SN:PGA_scaffold71579__1_contigs__length_45677 LN:45677 skipping SAM header line: @SQ SN:PGA_scaffold71639__1_contigs__length_32497 LN:32497 skipping SAM header line: @SQ SN:PGA_scaffold71656__1_contigs__length_30617 LN:30617 skipping SAM header line: @SQ SN:PGA_scaffold71660__1_contigs__length_43947 LN:43947 skipping SAM header line: @SQ SN:PGA_scaffold71671__1_contigs__length_34003 LN:34003 skipping SAM header line: @SQ SN:PGA_scaffold71686__1_contigs__length_54590 LN:54590 skipping SAM header line: @SQ SN:PGA_scaffold71700__1_contigs__length_34568 LN:34568 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold71876__1_contigs__length_78251 LN:78251 skipping SAM header line: @SQ SN:PGA_scaffold71891__1_contigs__length_32235 LN:32235 skipping SAM header line: @SQ SN:PGA_scaffold71897__1_contigs__length_31037 LN:31037 skipping SAM header line: @SQ SN:PGA_scaffold71912__1_contigs__length_30188 LN:30188 skipping SAM header line: @SQ SN:PGA_scaffold71942__1_contigs__length_31503 LN:31503 skipping SAM header line: @SQ SN:PGA_scaffold71943__1_contigs__length_31970 LN:31970 skipping SAM header line: @SQ SN:PGA_scaffold71993__1_contigs__length_39379 LN:39379 skipping SAM header line: @SQ SN:PGA_scaffold72048__1_contigs__length_41282 LN:41282 skipping SAM header line: @SQ SN:PGA_scaffold72054__1_contigs__length_45500 LN:45500 skipping SAM header line: @SQ SN:PGA_scaffold72055__1_contigs__length_38448 LN:38448 skipping SAM header line: @SQ SN:PGA_scaffold72064__1_contigs__length_49224 LN:49224 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold72225__1_contigs__length_34829 LN:34829 skipping SAM header line: @SQ SN:PGA_scaffold72252__1_contigs__length_37979 LN:37979 skipping SAM header line: @SQ SN:PGA_scaffold72282__1_contigs__length_53999 LN:53999 skipping SAM header line: @SQ SN:PGA_scaffold72348__1_contigs__length_34970 LN:34970 skipping SAM header line: @SQ SN:PGA_scaffold72363__1_contigs__length_51108 LN:51108 skipping SAM header line: @SQ SN:PGA_scaffold72392__1_contigs__length_64631 LN:64631 skipping SAM header line: @SQ SN:PGA_scaffold72407__1_contigs__length_43790 LN:43790 skipping SAM header line: @SQ SN:PGA_scaffold72408__1_contigs__length_33946 LN:33946 skipping SAM header line: @SQ SN:PGA_scaffold72417__1_contigs__length_30564 LN:30564 skipping SAM header line: @SQ SN:PGA_scaffold72453__1_contigs__length_31506 LN:31506 skipping SAM header line: @SQ SN:PGA_scaffold72480__1_contigs__length_68331 LN:68331 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold72971__1_contigs__length_35934 LN:35934 skipping SAM header line: @SQ SN:PGA_scaffold73010__1_contigs__length_40655 LN:40655 skipping SAM header line: @SQ SN:PGA_scaffold73051__1_contigs__length_30566 LN:30566 skipping SAM header line: @SQ SN:PGA_scaffold73063__1_contigs__length_31134 LN:31134 skipping SAM header line: @SQ SN:PGA_scaffold73106__1_contigs__length_43715 LN:43715 skipping SAM header line: @SQ SN:PGA_scaffold73248__1_contigs__length_33079 LN:33079 skipping SAM header line: @SQ SN:PGA_scaffold73249__1_contigs__length_30228 LN:30228 skipping SAM header line: @SQ SN:PGA_scaffold73264__1_contigs__length_37818 LN:37818 skipping SAM header line: @SQ SN:PGA_scaffold74017__1_contigs__length_32278 LN:32278 skipping SAM header line: @SQ SN:PGA_scaffold74018__1_contigs__length_32278 LN:32278 skipping SAM header line: @SQ SN:PGA_scaffold76166__1_contigs__length_39785 LN:39785 skipping SAM header line: @SQ 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LN:31617 skipping SAM header line: @SQ SN:PGA_scaffold119740__1_contigs__length_31617 LN:31617 skipping SAM header line: @SQ SN:PGA_scaffold120239__1_contigs__length_36781 LN:36781 skipping SAM header line: @SQ SN:PGA_scaffold120240__1_contigs__length_36781 LN:36781 skipping SAM header line: @SQ SN:PGA_scaffold120241__1_contigs__length_36781 LN:36781 skipping SAM header line: @SQ SN:PGA_scaffold120242__1_contigs__length_36781 LN:36781 skipping SAM header line: @SQ SN:PGA_scaffold120243__1_contigs__length_49726 LN:49726 skipping SAM header line: @SQ SN:PGA_scaffold120244__1_contigs__length_49726 LN:49726 skipping SAM header line: @SQ SN:PGA_scaffold120245__1_contigs__length_49726 LN:49726 skipping SAM header line: @SQ SN:PGA_scaffold120246__1_contigs__length_49726 LN:49726 skipping SAM header line: @SQ SN:PGA_scaffold154514__1_contigs__length_32931 LN:32931 skipping SAM header line: @SQ SN:PGA_scaffold154515__1_contigs__length_33776 LN:33776 skipping SAM header line: @SQ 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LN:2213805 skipping SAM header line: @SQ SN:PGA_scaffold313646__4_contigs__length_6274882 LN:6274882 skipping SAM header line: @SQ SN:PGA_scaffold313647__2_contigs__length_5689983 LN:5689983 skipping SAM header line: @SQ SN:PGA_scaffold313648__2_contigs__length_3786466 LN:3786466 skipping SAM header line: @SQ SN:PGA_scaffold313649__2_contigs__length_2716785 LN:2716785 skipping SAM header line: @PG ID:Bismark VN:v0.19.0 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64 --samtools_path /gscratch/srlab/programs/samtools-1.9 --score_min L,0,-1.2 -I 60 -p 28 --genome /gscratch/srlab/strigg/data/Pgenr/Pgenr_070_30K/ -1 /gscratch/srlab/strigg/data/Pgenr/FASTQS/data_for_genome_comparison/EPI-119_S31_L005_R1_001_val_1.fq.gz -2 /gscratch/srlab/strigg/data/Pgenr/FASTQS/data_for_genome_comparison/EPI-119_S31_L005_R2_001_val_2.fq.gz -o /gscratch/srlab/strigg/analyses/20190214_30K" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed 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11500000 Processed lines: 11500000 Processed lines: 12000000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12500000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12000000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 13000000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Processed lines: 12500000 Finished processing child process. Exiting.. Processed lines: 12500000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Processed lines: 13000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Now waiting for all child processes to complete Merging individual splitting reports into overall report: '/gscratch/srlab/strigg/analyses/20190214_30K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: Processed 13039685 lines in total Total number of methylation call strings processed: 26079370 Final Cytosine Methylation Report ================================= Total number of C's analysed: 310042817 Total methylated C's in CpG context: 9991454 Total methylated C's in CHG context: 1473467 Total methylated C's in CHH context: 3407120 Total C to T conversions in CpG context: 33514721 Total C to T conversions in CHG context: 62516201 Total C to T conversions in CHH context: 199139854 C methylated in CpG context: 23.0% C methylated in CHG context: 2.3% C methylated in CHH context: 1.7% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 101 Maximum read length of Read 2: 99 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 101 Maximum read length of Read 2: 99 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >/gscratch/srlab/strigg/analyses/20190214_30K/< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 2G Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: Writing bedGraph to file: EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Changed directory to /gscratch/srlab/strigg/analyses/20190214_30K/ The genome of interest was specified to contain gazillions of chromosomes or scaffolds. Merging all input files and sorting everything in memory instead of writing out individual chromosome files... Writing all merged methylation calls to temp file EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz.methylation_calls.merged Finished writing methylation calls from CpG_OT_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt to merged temp file Finished writing methylation calls from CpG_OB_EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt to merged temp file Sorting input file EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz.methylation_calls.merged by positions (using -S of 2G) Successfully deleted the temporary input file EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz.methylation_calls.merged Finished BedGraph conversion ... Checking file >>/gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... ...passed! Writing result file containing methylation information for C in CpG context from the original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the original bottom strand to /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:PGA_scaffold1__77_contigs__length_89643857 LN:89643857 skipping SAM header line: @SQ SN:PGA_scaffold2__36_contigs__length_69596280 LN:69596280 skipping SAM header line: @SQ SN:PGA_scaffold3__111_contigs__length_57743597 LN:57743597 skipping SAM header line: @SQ SN:PGA_scaffold4__129_contigs__length_65288255 LN:65288255 skipping SAM header line: @SQ SN:PGA_scaffold5__109_contigs__length_67248332 LN:67248332 skipping SAM header line: @SQ SN:PGA_scaffold6__104_contigs__length_61759565 LN:61759565 skipping SAM header line: @SQ SN:PGA_scaffold7__69_contigs__length_43120122 LN:43120122 skipping SAM header line: @SQ SN:PGA_scaffold8__63_contigs__length_61151155 LN:61151155 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold48890__1_contigs__length_36820 LN:36820 skipping SAM header line: @SQ SN:PGA_scaffold49049__1_contigs__length_44862 LN:44862 skipping SAM header line: @SQ SN:PGA_scaffold49052__1_contigs__length_30066 LN:30066 skipping SAM header line: @SQ SN:PGA_scaffold49140__1_contigs__length_41465 LN:41465 skipping SAM header line: @SQ SN:PGA_scaffold49269__1_contigs__length_58629 LN:58629 skipping SAM header line: @SQ SN:PGA_scaffold49272__1_contigs__length_48462 LN:48462 skipping SAM header line: @SQ SN:PGA_scaffold49286__1_contigs__length_32480 LN:32480 skipping SAM header line: @SQ SN:PGA_scaffold49327__1_contigs__length_31386 LN:31386 skipping SAM header line: @SQ SN:PGA_scaffold49337__1_contigs__length_44952 LN:44952 skipping SAM header line: @SQ SN:PGA_scaffold49402__1_contigs__length_32985 LN:32985 skipping SAM header line: @SQ SN:PGA_scaffold49433__1_contigs__length_36204 LN:36204 skipping SAM header line: @SQ SN:PGA_scaffold49448__1_contigs__length_40249 LN:40249 skipping SAM header line: @SQ SN:PGA_scaffold49600__1_contigs__length_30811 LN:30811 skipping SAM header line: @SQ SN:PGA_scaffold49609__1_contigs__length_30653 LN:30653 skipping SAM header line: @SQ SN:PGA_scaffold49622__1_contigs__length_34505 LN:34505 skipping SAM header line: @SQ SN:PGA_scaffold50268__1_contigs__length_48869 LN:48869 skipping SAM header line: @SQ SN:PGA_scaffold50335__1_contigs__length_43040 LN:43040 skipping SAM header line: @SQ SN:PGA_scaffold50414__1_contigs__length_43015 LN:43015 skipping SAM header line: @SQ SN:PGA_scaffold50424__1_contigs__length_37325 LN:37325 skipping SAM header line: @SQ SN:PGA_scaffold50725__1_contigs__length_39725 LN:39725 skipping SAM header line: @SQ SN:PGA_scaffold51021__1_contigs__length_35521 LN:35521 skipping SAM header line: @SQ SN:PGA_scaffold51544__1_contigs__length_32260 LN:32260 skipping SAM header line: @SQ SN:PGA_scaffold51684__1_contigs__length_32586 LN:32586 skipping SAM header line: @SQ SN:PGA_scaffold51827__1_contigs__length_44668 LN:44668 skipping SAM header line: @SQ SN:PGA_scaffold51925__1_contigs__length_33625 LN:33625 skipping SAM header line: @SQ SN:PGA_scaffold52047__1_contigs__length_52540 LN:52540 skipping SAM header line: @SQ SN:PGA_scaffold52050__1_contigs__length_46830 LN:46830 skipping SAM header line: @SQ SN:PGA_scaffold52113__1_contigs__length_31958 LN:31958 skipping SAM header line: @SQ SN:PGA_scaffold52128__1_contigs__length_39877 LN:39877 skipping SAM header line: @SQ SN:PGA_scaffold52199__1_contigs__length_32611 LN:32611 skipping SAM header line: @SQ SN:PGA_scaffold52211__1_contigs__length_36968 LN:36968 skipping SAM header line: @SQ SN:PGA_scaffold52215__1_contigs__length_42996 LN:42996 skipping SAM header line: @SQ SN:PGA_scaffold52222__1_contigs__length_39311 LN:39311 skipping SAM header line: @SQ SN:PGA_scaffold52266__1_contigs__length_35673 LN:35673 skipping SAM header line: @SQ SN:PGA_scaffold52328__1_contigs__length_39984 LN:39984 skipping SAM header line: @SQ SN:PGA_scaffold52349__1_contigs__length_37106 LN:37106 skipping SAM header line: @SQ SN:PGA_scaffold52350__1_contigs__length_45781 LN:45781 skipping SAM header line: @SQ SN:PGA_scaffold52359__1_contigs__length_33365 LN:33365 skipping SAM header line: @SQ SN:PGA_scaffold52387__1_contigs__length_40531 LN:40531 skipping SAM header line: @SQ SN:PGA_scaffold52390__1_contigs__length_35773 LN:35773 skipping SAM header line: @SQ SN:PGA_scaffold52392__1_contigs__length_46240 LN:46240 skipping SAM header line: @SQ SN:PGA_scaffold52393__1_contigs__length_36780 LN:36780 skipping SAM header line: @SQ SN:PGA_scaffold52394__1_contigs__length_53108 LN:53108 skipping SAM header line: @SQ SN:PGA_scaffold52396__1_contigs__length_36505 LN:36505 skipping SAM header line: @SQ SN:PGA_scaffold52869__1_contigs__length_35263 LN:35263 skipping SAM header line: @SQ SN:PGA_scaffold52872__1_contigs__length_34839 LN:34839 skipping SAM header line: @SQ SN:PGA_scaffold52892__1_contigs__length_39173 LN:39173 skipping SAM header line: @SQ SN:PGA_scaffold52893__1_contigs__length_36559 LN:36559 skipping SAM header line: @SQ SN:PGA_scaffold52914__1_contigs__length_54588 LN:54588 skipping SAM header line: @SQ SN:PGA_scaffold52916__1_contigs__length_30509 LN:30509 skipping SAM header line: @SQ SN:PGA_scaffold52973__1_contigs__length_33821 LN:33821 skipping SAM header line: @SQ SN:PGA_scaffold52979__1_contigs__length_30521 LN:30521 skipping SAM header line: @SQ SN:PGA_scaffold53021__1_contigs__length_37828 LN:37828 skipping SAM header line: @SQ SN:PGA_scaffold53196__1_contigs__length_30707 LN:30707 skipping SAM header line: @SQ SN:PGA_scaffold53343__1_contigs__length_31364 LN:31364 skipping SAM header line: @SQ SN:PGA_scaffold53591__1_contigs__length_57201 LN:57201 skipping SAM header line: @SQ SN:PGA_scaffold53633__1_contigs__length_37396 LN:37396 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold53990__1_contigs__length_50335 LN:50335 skipping SAM header line: @SQ SN:PGA_scaffold53991__1_contigs__length_51016 LN:51016 skipping SAM header line: @SQ SN:PGA_scaffold53992__1_contigs__length_30819 LN:30819 skipping SAM header line: @SQ SN:PGA_scaffold53994__1_contigs__length_45288 LN:45288 skipping SAM header line: @SQ SN:PGA_scaffold53995__1_contigs__length_36326 LN:36326 skipping SAM header line: @SQ SN:PGA_scaffold54015__1_contigs__length_30408 LN:30408 skipping SAM header line: @SQ SN:PGA_scaffold54205__1_contigs__length_38797 LN:38797 skipping SAM header line: @SQ SN:PGA_scaffold54342__1_contigs__length_60814 LN:60814 skipping SAM header line: @SQ SN:PGA_scaffold54901__1_contigs__length_37309 LN:37309 skipping SAM header line: @SQ SN:PGA_scaffold54965__1_contigs__length_38374 LN:38374 skipping SAM header line: @SQ SN:PGA_scaffold54999__1_contigs__length_31291 LN:31291 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold55378__1_contigs__length_34882 LN:34882 skipping SAM header line: @SQ SN:PGA_scaffold55381__1_contigs__length_30600 LN:30600 skipping SAM header line: @SQ SN:PGA_scaffold55711__1_contigs__length_39787 LN:39787 skipping SAM header line: @SQ SN:PGA_scaffold55714__1_contigs__length_36372 LN:36372 skipping SAM header line: @SQ SN:PGA_scaffold55745__1_contigs__length_39718 LN:39718 skipping SAM header line: @SQ SN:PGA_scaffold55747__1_contigs__length_42692 LN:42692 skipping SAM header line: @SQ SN:PGA_scaffold55855__1_contigs__length_34489 LN:34489 skipping SAM header line: @SQ SN:PGA_scaffold55860__1_contigs__length_50662 LN:50662 skipping SAM header line: @SQ SN:PGA_scaffold55979__1_contigs__length_33616 LN:33616 skipping SAM header line: @SQ SN:PGA_scaffold56091__1_contigs__length_39544 LN:39544 skipping SAM header line: @SQ SN:PGA_scaffold56365__1_contigs__length_56315 LN:56315 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold57070__1_contigs__length_35859 LN:35859 skipping SAM header line: @SQ SN:PGA_scaffold57215__1_contigs__length_43048 LN:43048 skipping SAM header line: @SQ SN:PGA_scaffold57298__1_contigs__length_31957 LN:31957 skipping SAM header line: @SQ SN:PGA_scaffold57301__1_contigs__length_34452 LN:34452 skipping SAM header line: @SQ SN:PGA_scaffold57398__1_contigs__length_30770 LN:30770 skipping SAM header line: @SQ SN:PGA_scaffold57419__1_contigs__length_56657 LN:56657 skipping SAM header line: @SQ SN:PGA_scaffold57435__1_contigs__length_39348 LN:39348 skipping SAM header line: @SQ SN:PGA_scaffold57479__1_contigs__length_34907 LN:34907 skipping SAM header line: @SQ SN:PGA_scaffold57490__1_contigs__length_31663 LN:31663 skipping SAM header line: @SQ SN:PGA_scaffold57492__1_contigs__length_41689 LN:41689 skipping SAM header line: @SQ SN:PGA_scaffold57510__1_contigs__length_39178 LN:39178 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold57901__1_contigs__length_33405 LN:33405 skipping SAM header line: @SQ SN:PGA_scaffold58076__1_contigs__length_32943 LN:32943 skipping SAM header line: @SQ SN:PGA_scaffold58238__1_contigs__length_68373 LN:68373 skipping SAM header line: @SQ SN:PGA_scaffold58264__1_contigs__length_33661 LN:33661 skipping SAM header line: @SQ SN:PGA_scaffold58284__1_contigs__length_33826 LN:33826 skipping SAM header line: @SQ SN:PGA_scaffold58361__1_contigs__length_52751 LN:52751 skipping SAM header line: @SQ SN:PGA_scaffold58366__1_contigs__length_37326 LN:37326 skipping SAM header line: @SQ SN:PGA_scaffold58382__1_contigs__length_41713 LN:41713 skipping SAM header line: @SQ SN:PGA_scaffold58383__1_contigs__length_44366 LN:44366 skipping SAM header line: @SQ SN:PGA_scaffold58388__1_contigs__length_32850 LN:32850 skipping SAM header line: @SQ SN:PGA_scaffold58393__1_contigs__length_33434 LN:33434 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold58676__1_contigs__length_42740 LN:42740 skipping SAM header line: @SQ SN:PGA_scaffold58700__1_contigs__length_38161 LN:38161 skipping SAM header line: @SQ SN:PGA_scaffold58712__1_contigs__length_43972 LN:43972 skipping SAM header line: @SQ SN:PGA_scaffold58749__1_contigs__length_42553 LN:42553 skipping SAM header line: @SQ SN:PGA_scaffold58976__1_contigs__length_32077 LN:32077 skipping SAM header line: @SQ SN:PGA_scaffold58984__1_contigs__length_30766 LN:30766 skipping SAM header line: @SQ SN:PGA_scaffold59041__1_contigs__length_49211 LN:49211 skipping SAM header line: @SQ SN:PGA_scaffold59054__1_contigs__length_30586 LN:30586 skipping SAM header line: @SQ SN:PGA_scaffold59056__1_contigs__length_33360 LN:33360 skipping SAM header line: @SQ SN:PGA_scaffold59166__1_contigs__length_30875 LN:30875 skipping SAM header line: @SQ SN:PGA_scaffold59171__1_contigs__length_43122 LN:43122 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold59687__1_contigs__length_34469 LN:34469 skipping SAM header line: @SQ SN:PGA_scaffold59721__1_contigs__length_42511 LN:42511 skipping SAM header line: @SQ SN:PGA_scaffold59723__1_contigs__length_39806 LN:39806 skipping SAM header line: @SQ SN:PGA_scaffold59727__1_contigs__length_31211 LN:31211 skipping SAM header line: @SQ SN:PGA_scaffold59729__1_contigs__length_32301 LN:32301 skipping SAM header line: @SQ SN:PGA_scaffold59750__1_contigs__length_36894 LN:36894 skipping SAM header line: @SQ SN:PGA_scaffold59858__1_contigs__length_39712 LN:39712 skipping SAM header line: @SQ SN:PGA_scaffold59872__1_contigs__length_51346 LN:51346 skipping SAM header line: @SQ SN:PGA_scaffold59907__1_contigs__length_30865 LN:30865 skipping SAM header line: @SQ SN:PGA_scaffold59933__1_contigs__length_43116 LN:43116 skipping SAM header line: @SQ SN:PGA_scaffold59943__1_contigs__length_33685 LN:33685 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold61037__1_contigs__length_31360 LN:31360 skipping SAM header line: @SQ SN:PGA_scaffold61040__1_contigs__length_32690 LN:32690 skipping SAM header line: @SQ SN:PGA_scaffold61046__1_contigs__length_41502 LN:41502 skipping SAM header line: @SQ SN:PGA_scaffold61063__1_contigs__length_47605 LN:47605 skipping SAM header line: @SQ SN:PGA_scaffold61072__1_contigs__length_33361 LN:33361 skipping SAM header line: @SQ SN:PGA_scaffold61096__1_contigs__length_30606 LN:30606 skipping SAM header line: @SQ SN:PGA_scaffold61099__1_contigs__length_49058 LN:49058 skipping SAM header line: @SQ SN:PGA_scaffold61103__1_contigs__length_37635 LN:37635 skipping SAM header line: @SQ SN:PGA_scaffold61168__1_contigs__length_30806 LN:30806 skipping SAM header line: @SQ SN:PGA_scaffold61190__1_contigs__length_38143 LN:38143 skipping SAM header line: @SQ SN:PGA_scaffold61242__1_contigs__length_43427 LN:43427 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold61392__1_contigs__length_57213 LN:57213 skipping SAM header line: @SQ SN:PGA_scaffold61445__1_contigs__length_33748 LN:33748 skipping SAM header line: @SQ SN:PGA_scaffold61589__1_contigs__length_43181 LN:43181 skipping SAM header line: @SQ SN:PGA_scaffold61592__1_contigs__length_35695 LN:35695 skipping SAM header line: @SQ SN:PGA_scaffold61615__1_contigs__length_40934 LN:40934 skipping SAM header line: @SQ SN:PGA_scaffold61619__1_contigs__length_33825 LN:33825 skipping SAM header line: @SQ SN:PGA_scaffold61628__1_contigs__length_48494 LN:48494 skipping SAM header line: @SQ SN:PGA_scaffold61636__1_contigs__length_47931 LN:47931 skipping SAM header line: @SQ SN:PGA_scaffold61640__1_contigs__length_57604 LN:57604 skipping SAM header line: @SQ SN:PGA_scaffold61646__1_contigs__length_55250 LN:55250 skipping SAM header line: @SQ SN:PGA_scaffold61650__1_contigs__length_31696 LN:31696 skipping SAM header line: @SQ SN:PGA_scaffold61652__1_contigs__length_58886 LN:58886 skipping SAM header line: @SQ SN:PGA_scaffold61705__1_contigs__length_34844 LN:34844 skipping SAM header line: @SQ SN:PGA_scaffold61706__1_contigs__length_34820 LN:34820 skipping SAM header line: @SQ SN:PGA_scaffold61707__1_contigs__length_41227 LN:41227 skipping SAM header line: @SQ SN:PGA_scaffold61777__1_contigs__length_45224 LN:45224 skipping SAM header line: @SQ SN:PGA_scaffold61840__1_contigs__length_33628 LN:33628 skipping SAM header line: @SQ SN:PGA_scaffold61847__1_contigs__length_83310 LN:83310 skipping SAM header line: @SQ SN:PGA_scaffold61852__1_contigs__length_31557 LN:31557 skipping SAM header line: @SQ SN:PGA_scaffold61853__1_contigs__length_48778 LN:48778 skipping SAM header line: @SQ SN:PGA_scaffold61879__1_contigs__length_44422 LN:44422 skipping SAM header line: @SQ SN:PGA_scaffold61884__1_contigs__length_46735 LN:46735 skipping SAM header line: @SQ SN:PGA_scaffold61888__1_contigs__length_43188 LN:43188 skipping SAM header line: @SQ SN:PGA_scaffold61897__1_contigs__length_41371 LN:41371 skipping SAM header line: @SQ SN:PGA_scaffold61982__1_contigs__length_37118 LN:37118 skipping SAM header line: @SQ SN:PGA_scaffold62098__1_contigs__length_77920 LN:77920 skipping SAM header line: @SQ SN:PGA_scaffold62138__1_contigs__length_33334 LN:33334 skipping SAM header line: @SQ SN:PGA_scaffold62168__1_contigs__length_43227 LN:43227 skipping SAM header line: @SQ SN:PGA_scaffold62186__1_contigs__length_43446 LN:43446 skipping SAM header line: @SQ SN:PGA_scaffold62192__1_contigs__length_56278 LN:56278 skipping SAM header line: @SQ SN:PGA_scaffold62198__1_contigs__length_38255 LN:38255 skipping SAM header line: @SQ SN:PGA_scaffold62206__1_contigs__length_67303 LN:67303 skipping SAM header line: @SQ SN:PGA_scaffold62224__1_contigs__length_74638 LN:74638 skipping SAM header line: @SQ SN:PGA_scaffold62226__1_contigs__length_86027 LN:86027 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold62542__1_contigs__length_32921 LN:32921 skipping SAM header line: @SQ SN:PGA_scaffold62544__1_contigs__length_41829 LN:41829 skipping SAM header line: @SQ SN:PGA_scaffold62591__1_contigs__length_64464 LN:64464 skipping SAM header line: @SQ SN:PGA_scaffold62601__1_contigs__length_36053 LN:36053 skipping SAM header line: @SQ SN:PGA_scaffold62602__1_contigs__length_30153 LN:30153 skipping SAM header line: @SQ SN:PGA_scaffold62661__1_contigs__length_34648 LN:34648 skipping SAM header line: @SQ SN:PGA_scaffold62687__1_contigs__length_56019 LN:56019 skipping SAM header line: @SQ SN:PGA_scaffold62752__1_contigs__length_33202 LN:33202 skipping SAM header line: @SQ SN:PGA_scaffold62854__1_contigs__length_47148 LN:47148 skipping SAM header line: @SQ SN:PGA_scaffold62862__1_contigs__length_31728 LN:31728 skipping SAM header line: @SQ SN:PGA_scaffold62865__1_contigs__length_39731 LN:39731 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold62982__1_contigs__length_51892 LN:51892 skipping SAM header line: @SQ SN:PGA_scaffold63008__1_contigs__length_40245 LN:40245 skipping SAM header line: @SQ SN:PGA_scaffold63022__1_contigs__length_31832 LN:31832 skipping SAM header line: @SQ SN:PGA_scaffold63033__1_contigs__length_64137 LN:64137 skipping SAM header line: @SQ SN:PGA_scaffold63057__1_contigs__length_30046 LN:30046 skipping SAM header line: @SQ SN:PGA_scaffold63059__1_contigs__length_30993 LN:30993 skipping SAM header line: @SQ SN:PGA_scaffold63073__1_contigs__length_59843 LN:59843 skipping SAM header line: @SQ SN:PGA_scaffold63076__1_contigs__length_65566 LN:65566 skipping SAM header line: @SQ SN:PGA_scaffold63085__1_contigs__length_46291 LN:46291 skipping SAM header line: @SQ SN:PGA_scaffold63090__1_contigs__length_43963 LN:43963 skipping SAM header line: @SQ SN:PGA_scaffold63101__1_contigs__length_45043 LN:45043 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold63286__1_contigs__length_64799 LN:64799 skipping SAM header line: @SQ SN:PGA_scaffold63295__1_contigs__length_73242 LN:73242 skipping SAM header line: @SQ SN:PGA_scaffold63327__1_contigs__length_41823 LN:41823 skipping SAM header line: @SQ SN:PGA_scaffold63330__1_contigs__length_32542 LN:32542 skipping SAM header line: @SQ SN:PGA_scaffold63338__1_contigs__length_30825 LN:30825 skipping SAM header line: @SQ SN:PGA_scaffold63480__1_contigs__length_39812 LN:39812 skipping SAM header line: @SQ SN:PGA_scaffold63504__1_contigs__length_83511 LN:83511 skipping SAM header line: @SQ SN:PGA_scaffold63543__1_contigs__length_67322 LN:67322 skipping SAM header line: @SQ SN:PGA_scaffold63594__1_contigs__length_42878 LN:42878 skipping SAM header line: @SQ SN:PGA_scaffold63635__1_contigs__length_31389 LN:31389 skipping SAM header line: @SQ SN:PGA_scaffold63681__1_contigs__length_35508 LN:35508 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold65693__1_contigs__length_34648 LN:34648 skipping SAM header line: @SQ SN:PGA_scaffold65737__1_contigs__length_37231 LN:37231 skipping SAM header line: @SQ SN:PGA_scaffold65765__1_contigs__length_39217 LN:39217 skipping SAM header line: @SQ SN:PGA_scaffold65777__1_contigs__length_32724 LN:32724 skipping SAM header line: @SQ SN:PGA_scaffold65781__1_contigs__length_42302 LN:42302 skipping SAM header line: @SQ SN:PGA_scaffold65786__1_contigs__length_56597 LN:56597 skipping SAM header line: @SQ SN:PGA_scaffold65807__1_contigs__length_35379 LN:35379 skipping SAM header line: @SQ SN:PGA_scaffold65809__1_contigs__length_36632 LN:36632 skipping SAM header line: @SQ SN:PGA_scaffold65815__1_contigs__length_49308 LN:49308 skipping SAM header line: @SQ SN:PGA_scaffold65846__1_contigs__length_33986 LN:33986 skipping SAM header line: @SQ SN:PGA_scaffold65851__1_contigs__length_44621 LN:44621 skipping SAM header line: @SQ SN:PGA_scaffold65866__1_contigs__length_90471 LN:90471 skipping SAM header line: @SQ SN:PGA_scaffold65911__1_contigs__length_48303 LN:48303 skipping SAM header line: @SQ SN:PGA_scaffold65912__1_contigs__length_34561 LN:34561 skipping SAM header line: @SQ SN:PGA_scaffold65945__1_contigs__length_40559 LN:40559 skipping SAM header line: @SQ SN:PGA_scaffold65949__1_contigs__length_35336 LN:35336 skipping SAM header line: @SQ SN:PGA_scaffold65954__1_contigs__length_39725 LN:39725 skipping SAM header line: @SQ SN:PGA_scaffold65967__1_contigs__length_36096 LN:36096 skipping SAM header line: @SQ SN:PGA_scaffold65987__1_contigs__length_52560 LN:52560 skipping SAM header line: @SQ SN:PGA_scaffold66001__1_contigs__length_39013 LN:39013 skipping SAM header line: @SQ SN:PGA_scaffold66037__1_contigs__length_37904 LN:37904 skipping SAM header line: @SQ SN:PGA_scaffold66060__1_contigs__length_47051 LN:47051 skipping SAM header line: @SQ SN:PGA_scaffold66062__1_contigs__length_35720 LN:35720 skipping SAM header line: @SQ SN:PGA_scaffold66079__1_contigs__length_37644 LN:37644 skipping SAM header line: @SQ SN:PGA_scaffold66102__1_contigs__length_38669 LN:38669 skipping SAM header line: @SQ SN:PGA_scaffold66132__1_contigs__length_39192 LN:39192 skipping SAM header line: @SQ SN:PGA_scaffold66172__1_contigs__length_62844 LN:62844 skipping SAM header line: @SQ SN:PGA_scaffold66173__1_contigs__length_34319 LN:34319 skipping SAM header line: @SQ SN:PGA_scaffold66178__1_contigs__length_49601 LN:49601 skipping SAM header line: @SQ SN:PGA_scaffold66186__1_contigs__length_45928 LN:45928 skipping SAM header line: @SQ SN:PGA_scaffold66196__1_contigs__length_53841 LN:53841 skipping SAM header line: @SQ SN:PGA_scaffold66212__1_contigs__length_32503 LN:32503 skipping SAM header line: @SQ SN:PGA_scaffold66276__1_contigs__length_39820 LN:39820 skipping SAM header line: @SQ SN:PGA_scaffold66293__1_contigs__length_33304 LN:33304 skipping SAM header line: @SQ SN:PGA_scaffold66318__1_contigs__length_45619 LN:45619 skipping SAM header line: @SQ SN:PGA_scaffold66338__1_contigs__length_30756 LN:30756 skipping SAM header line: @SQ SN:PGA_scaffold66399__1_contigs__length_42456 LN:42456 skipping SAM header line: @SQ SN:PGA_scaffold66419__1_contigs__length_49715 LN:49715 skipping SAM header line: @SQ SN:PGA_scaffold66434__1_contigs__length_46589 LN:46589 skipping SAM header line: @SQ SN:PGA_scaffold66452__1_contigs__length_42482 LN:42482 skipping SAM header line: @SQ SN:PGA_scaffold66481__1_contigs__length_32127 LN:32127 skipping SAM header line: @SQ SN:PGA_scaffold66513__1_contigs__length_33853 LN:33853 skipping SAM header line: @SQ SN:PGA_scaffold66519__1_contigs__length_33851 LN:33851 skipping SAM header line: @SQ SN:PGA_scaffold66554__1_contigs__length_46378 LN:46378 skipping SAM header line: @SQ SN:PGA_scaffold66557__1_contigs__length_39298 LN:39298 skipping SAM header line: @SQ SN:PGA_scaffold66594__1_contigs__length_45007 LN:45007 skipping SAM header line: @SQ SN:PGA_scaffold66641__1_contigs__length_41123 LN:41123 skipping SAM header line: @SQ SN:PGA_scaffold66642__1_contigs__length_33139 LN:33139 skipping SAM header line: @SQ SN:PGA_scaffold66656__1_contigs__length_57103 LN:57103 skipping SAM header line: @SQ SN:PGA_scaffold66666__1_contigs__length_58206 LN:58206 skipping SAM header line: @SQ SN:PGA_scaffold66674__1_contigs__length_30853 LN:30853 skipping SAM header line: @SQ SN:PGA_scaffold66682__1_contigs__length_34382 LN:34382 skipping SAM header line: @SQ SN:PGA_scaffold66713__1_contigs__length_84867 LN:84867 skipping SAM header line: @SQ SN:PGA_scaffold66717__1_contigs__length_43668 LN:43668 skipping SAM header line: @SQ SN:PGA_scaffold66721__1_contigs__length_30932 LN:30932 skipping SAM header line: @SQ SN:PGA_scaffold66729__1_contigs__length_32971 LN:32971 skipping SAM header line: @SQ SN:PGA_scaffold66737__1_contigs__length_35128 LN:35128 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold67214__1_contigs__length_49673 LN:49673 skipping SAM header line: @SQ SN:PGA_scaffold67280__1_contigs__length_31885 LN:31885 skipping SAM header line: @SQ SN:PGA_scaffold67294__1_contigs__length_50832 LN:50832 skipping SAM header line: @SQ SN:PGA_scaffold67337__1_contigs__length_52553 LN:52553 skipping SAM header line: @SQ SN:PGA_scaffold67346__1_contigs__length_32090 LN:32090 skipping SAM header line: @SQ SN:PGA_scaffold67399__1_contigs__length_33211 LN:33211 skipping SAM header line: @SQ SN:PGA_scaffold67407__1_contigs__length_73205 LN:73205 skipping SAM header line: @SQ SN:PGA_scaffold67409__1_contigs__length_30272 LN:30272 skipping SAM header line: @SQ SN:PGA_scaffold67460__1_contigs__length_63707 LN:63707 skipping SAM header line: @SQ SN:PGA_scaffold67469__1_contigs__length_34100 LN:34100 skipping SAM header line: @SQ SN:PGA_scaffold67491__1_contigs__length_36666 LN:36666 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold67633__1_contigs__length_49909 LN:49909 skipping SAM header line: @SQ SN:PGA_scaffold67641__1_contigs__length_35654 LN:35654 skipping SAM header line: @SQ SN:PGA_scaffold67666__1_contigs__length_32329 LN:32329 skipping SAM header line: @SQ SN:PGA_scaffold67687__1_contigs__length_62665 LN:62665 skipping SAM header line: @SQ SN:PGA_scaffold67702__1_contigs__length_30259 LN:30259 skipping SAM header line: @SQ SN:PGA_scaffold67709__1_contigs__length_32364 LN:32364 skipping SAM header line: @SQ SN:PGA_scaffold67745__1_contigs__length_41387 LN:41387 skipping SAM header line: @SQ SN:PGA_scaffold67754__1_contigs__length_46865 LN:46865 skipping SAM header line: @SQ SN:PGA_scaffold67781__1_contigs__length_42225 LN:42225 skipping SAM header line: @SQ SN:PGA_scaffold67782__1_contigs__length_31631 LN:31631 skipping SAM header line: @SQ SN:PGA_scaffold67794__1_contigs__length_36514 LN:36514 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold68053__1_contigs__length_31000 LN:31000 skipping SAM header line: @SQ SN:PGA_scaffold68092__1_contigs__length_33414 LN:33414 skipping SAM header line: @SQ SN:PGA_scaffold68150__1_contigs__length_50783 LN:50783 skipping SAM header line: @SQ SN:PGA_scaffold68157__1_contigs__length_34106 LN:34106 skipping SAM header line: @SQ SN:PGA_scaffold68184__1_contigs__length_41355 LN:41355 skipping SAM header line: @SQ SN:PGA_scaffold68193__1_contigs__length_46031 LN:46031 skipping SAM header line: @SQ SN:PGA_scaffold68205__1_contigs__length_30954 LN:30954 skipping SAM header line: @SQ SN:PGA_scaffold68252__1_contigs__length_37078 LN:37078 skipping SAM header line: @SQ SN:PGA_scaffold68273__1_contigs__length_46075 LN:46075 skipping SAM header line: @SQ SN:PGA_scaffold68274__1_contigs__length_63866 LN:63866 skipping SAM header line: @SQ SN:PGA_scaffold68332__1_contigs__length_52891 LN:52891 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold69000__1_contigs__length_31325 LN:31325 skipping SAM header line: @SQ SN:PGA_scaffold69031__1_contigs__length_49096 LN:49096 skipping SAM header line: @SQ SN:PGA_scaffold69064__1_contigs__length_30263 LN:30263 skipping SAM header line: @SQ SN:PGA_scaffold69066__1_contigs__length_41967 LN:41967 skipping SAM header line: @SQ SN:PGA_scaffold69075__1_contigs__length_43631 LN:43631 skipping SAM header line: @SQ SN:PGA_scaffold69088__1_contigs__length_46450 LN:46450 skipping SAM header line: @SQ SN:PGA_scaffold69098__1_contigs__length_35440 LN:35440 skipping SAM header line: @SQ SN:PGA_scaffold69099__1_contigs__length_56136 LN:56136 skipping SAM header line: @SQ SN:PGA_scaffold69107__1_contigs__length_36690 LN:36690 skipping SAM header line: @SQ SN:PGA_scaffold69130__1_contigs__length_52115 LN:52115 skipping SAM header line: @SQ SN:PGA_scaffold69149__1_contigs__length_34885 LN:34885 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold69439__1_contigs__length_41542 LN:41542 skipping SAM header line: @SQ SN:PGA_scaffold69451__1_contigs__length_39160 LN:39160 skipping SAM header line: @SQ SN:PGA_scaffold69459__1_contigs__length_45501 LN:45501 skipping SAM header line: @SQ SN:PGA_scaffold69477__1_contigs__length_38210 LN:38210 skipping SAM header line: @SQ SN:PGA_scaffold69479__1_contigs__length_32808 LN:32808 skipping SAM header line: @SQ SN:PGA_scaffold69484__1_contigs__length_31150 LN:31150 skipping SAM header line: @SQ SN:PGA_scaffold69495__1_contigs__length_53052 LN:53052 skipping SAM header line: @SQ SN:PGA_scaffold69529__1_contigs__length_33639 LN:33639 skipping SAM header line: @SQ SN:PGA_scaffold69555__1_contigs__length_42966 LN:42966 skipping SAM header line: @SQ SN:PGA_scaffold69556__1_contigs__length_33961 LN:33961 skipping SAM header line: @SQ SN:PGA_scaffold69619__1_contigs__length_30388 LN:30388 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold69807__1_contigs__length_45521 LN:45521 skipping SAM header line: @SQ SN:PGA_scaffold69829__1_contigs__length_34810 LN:34810 skipping SAM header line: @SQ SN:PGA_scaffold69869__1_contigs__length_52996 LN:52996 skipping SAM header line: @SQ SN:PGA_scaffold69890__1_contigs__length_34157 LN:34157 skipping SAM header line: @SQ SN:PGA_scaffold69901__1_contigs__length_33452 LN:33452 skipping SAM header line: @SQ SN:PGA_scaffold69924__1_contigs__length_44446 LN:44446 skipping SAM header line: @SQ SN:PGA_scaffold69935__1_contigs__length_32162 LN:32162 skipping SAM header line: @SQ SN:PGA_scaffold69949__1_contigs__length_34320 LN:34320 skipping SAM header line: @SQ SN:PGA_scaffold69958__1_contigs__length_74035 LN:74035 skipping SAM header line: @SQ SN:PGA_scaffold69967__1_contigs__length_33074 LN:33074 skipping SAM header line: @SQ SN:PGA_scaffold69970__1_contigs__length_36785 LN:36785 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold70204__1_contigs__length_36625 LN:36625 skipping SAM header line: @SQ SN:PGA_scaffold70218__1_contigs__length_31585 LN:31585 skipping SAM header line: @SQ SN:PGA_scaffold70228__1_contigs__length_47471 LN:47471 skipping SAM header line: @SQ SN:PGA_scaffold70241__1_contigs__length_36884 LN:36884 skipping SAM header line: @SQ SN:PGA_scaffold70287__1_contigs__length_50534 LN:50534 skipping SAM header line: @SQ SN:PGA_scaffold70301__1_contigs__length_33670 LN:33670 skipping SAM header line: @SQ SN:PGA_scaffold70359__1_contigs__length_31823 LN:31823 skipping SAM header line: @SQ SN:PGA_scaffold70409__1_contigs__length_30379 LN:30379 skipping SAM header line: @SQ SN:PGA_scaffold70448__1_contigs__length_39500 LN:39500 skipping SAM header line: @SQ SN:PGA_scaffold70468__1_contigs__length_51685 LN:51685 skipping SAM header line: @SQ SN:PGA_scaffold70474__1_contigs__length_50365 LN:50365 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold70650__1_contigs__length_36340 LN:36340 skipping SAM header line: @SQ SN:PGA_scaffold70652__1_contigs__length_42814 LN:42814 skipping SAM header line: @SQ SN:PGA_scaffold70653__1_contigs__length_42188 LN:42188 skipping SAM header line: @SQ SN:PGA_scaffold70667__1_contigs__length_31073 LN:31073 skipping SAM header line: @SQ SN:PGA_scaffold70744__1_contigs__length_41353 LN:41353 skipping SAM header line: @SQ SN:PGA_scaffold70751__1_contigs__length_35732 LN:35732 skipping SAM header line: @SQ SN:PGA_scaffold70770__1_contigs__length_37647 LN:37647 skipping SAM header line: @SQ SN:PGA_scaffold70785__1_contigs__length_35848 LN:35848 skipping SAM header line: @SQ SN:PGA_scaffold70788__1_contigs__length_34232 LN:34232 skipping SAM header line: @SQ SN:PGA_scaffold70791__1_contigs__length_36664 LN:36664 skipping SAM header line: @SQ SN:PGA_scaffold70793__1_contigs__length_31797 LN:31797 skipping SAM header line: @SQ SN:PGA_scaffold70795__1_contigs__length_35890 LN:35890 skipping SAM header line: @SQ SN:PGA_scaffold70814__1_contigs__length_47056 LN:47056 skipping SAM header line: @SQ SN:PGA_scaffold70837__1_contigs__length_33454 LN:33454 skipping SAM header line: @SQ SN:PGA_scaffold70839__1_contigs__length_41296 LN:41296 skipping SAM header line: @SQ SN:PGA_scaffold70880__1_contigs__length_31372 LN:31372 skipping SAM header line: @SQ SN:PGA_scaffold70894__1_contigs__length_47158 LN:47158 skipping SAM header line: @SQ SN:PGA_scaffold70911__1_contigs__length_31781 LN:31781 skipping SAM header line: @SQ SN:PGA_scaffold70919__1_contigs__length_35080 LN:35080 skipping SAM header line: @SQ SN:PGA_scaffold70967__1_contigs__length_30258 LN:30258 skipping SAM header line: @SQ SN:PGA_scaffold70990__1_contigs__length_44272 LN:44272 skipping SAM header line: @SQ SN:PGA_scaffold71003__1_contigs__length_32258 LN:32258 skipping SAM header line: @SQ SN:PGA_scaffold71016__1_contigs__length_52719 LN:52719 skipping SAM header line: @SQ SN:PGA_scaffold71023__1_contigs__length_42297 LN:42297 skipping SAM header line: @SQ SN:PGA_scaffold71024__1_contigs__length_37906 LN:37906 skipping SAM header line: @SQ SN:PGA_scaffold71080__1_contigs__length_35132 LN:35132 skipping SAM header line: @SQ SN:PGA_scaffold71087__1_contigs__length_36896 LN:36896 skipping SAM header line: @SQ SN:PGA_scaffold71091__1_contigs__length_33646 LN:33646 skipping SAM header line: @SQ SN:PGA_scaffold71150__1_contigs__length_30573 LN:30573 skipping SAM header line: @SQ SN:PGA_scaffold71155__1_contigs__length_32524 LN:32524 skipping SAM header line: @SQ SN:PGA_scaffold71157__1_contigs__length_46861 LN:46861 skipping SAM header line: @SQ SN:PGA_scaffold71196__1_contigs__length_54597 LN:54597 skipping SAM header line: @SQ SN:PGA_scaffold71206__1_contigs__length_30090 LN:30090 skipping SAM header line: @SQ SN:PGA_scaffold71207__1_contigs__length_51143 LN:51143 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold71455__1_contigs__length_57134 LN:57134 skipping SAM header line: @SQ SN:PGA_scaffold71488__1_contigs__length_33569 LN:33569 skipping SAM header line: @SQ SN:PGA_scaffold71520__1_contigs__length_33829 LN:33829 skipping SAM header line: @SQ SN:PGA_scaffold71528__1_contigs__length_39316 LN:39316 skipping SAM header line: @SQ SN:PGA_scaffold71579__1_contigs__length_45677 LN:45677 skipping SAM header line: @SQ SN:PGA_scaffold71639__1_contigs__length_32497 LN:32497 skipping SAM header line: @SQ SN:PGA_scaffold71656__1_contigs__length_30617 LN:30617 skipping SAM header line: @SQ SN:PGA_scaffold71660__1_contigs__length_43947 LN:43947 skipping SAM header line: @SQ SN:PGA_scaffold71671__1_contigs__length_34003 LN:34003 skipping SAM header line: @SQ SN:PGA_scaffold71686__1_contigs__length_54590 LN:54590 skipping SAM header line: @SQ SN:PGA_scaffold71700__1_contigs__length_34568 LN:34568 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold71876__1_contigs__length_78251 LN:78251 skipping SAM header line: @SQ SN:PGA_scaffold71891__1_contigs__length_32235 LN:32235 skipping SAM header line: @SQ SN:PGA_scaffold71897__1_contigs__length_31037 LN:31037 skipping SAM header line: @SQ SN:PGA_scaffold71912__1_contigs__length_30188 LN:30188 skipping SAM header line: @SQ SN:PGA_scaffold71942__1_contigs__length_31503 LN:31503 skipping SAM header line: @SQ SN:PGA_scaffold71943__1_contigs__length_31970 LN:31970 skipping SAM header line: @SQ SN:PGA_scaffold71993__1_contigs__length_39379 LN:39379 skipping SAM header line: @SQ SN:PGA_scaffold72048__1_contigs__length_41282 LN:41282 skipping SAM header line: @SQ SN:PGA_scaffold72054__1_contigs__length_45500 LN:45500 skipping SAM header line: @SQ SN:PGA_scaffold72055__1_contigs__length_38448 LN:38448 skipping SAM header line: @SQ SN:PGA_scaffold72064__1_contigs__length_49224 LN:49224 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold72225__1_contigs__length_34829 LN:34829 skipping SAM header line: @SQ SN:PGA_scaffold72252__1_contigs__length_37979 LN:37979 skipping SAM header line: @SQ SN:PGA_scaffold72282__1_contigs__length_53999 LN:53999 skipping SAM header line: @SQ SN:PGA_scaffold72348__1_contigs__length_34970 LN:34970 skipping SAM header line: @SQ SN:PGA_scaffold72363__1_contigs__length_51108 LN:51108 skipping SAM header line: @SQ SN:PGA_scaffold72392__1_contigs__length_64631 LN:64631 skipping SAM header line: @SQ SN:PGA_scaffold72407__1_contigs__length_43790 LN:43790 skipping SAM header line: @SQ SN:PGA_scaffold72408__1_contigs__length_33946 LN:33946 skipping SAM header line: @SQ SN:PGA_scaffold72417__1_contigs__length_30564 LN:30564 skipping SAM header line: @SQ SN:PGA_scaffold72453__1_contigs__length_31506 LN:31506 skipping SAM header line: @SQ SN:PGA_scaffold72480__1_contigs__length_68331 LN:68331 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold72971__1_contigs__length_35934 LN:35934 skipping SAM header line: @SQ SN:PGA_scaffold73010__1_contigs__length_40655 LN:40655 skipping SAM header line: @SQ SN:PGA_scaffold73051__1_contigs__length_30566 LN:30566 skipping SAM header line: @SQ SN:PGA_scaffold73063__1_contigs__length_31134 LN:31134 skipping SAM header line: @SQ SN:PGA_scaffold73106__1_contigs__length_43715 LN:43715 skipping SAM header line: @SQ SN:PGA_scaffold73248__1_contigs__length_33079 LN:33079 skipping SAM header line: @SQ SN:PGA_scaffold73249__1_contigs__length_30228 LN:30228 skipping SAM header line: @SQ SN:PGA_scaffold73264__1_contigs__length_37818 LN:37818 skipping SAM header line: @SQ SN:PGA_scaffold74017__1_contigs__length_32278 LN:32278 skipping SAM header line: @SQ SN:PGA_scaffold74018__1_contigs__length_32278 LN:32278 skipping SAM header line: @SQ SN:PGA_scaffold76166__1_contigs__length_39785 LN:39785 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold82845__1_contigs__length_39038 LN:39038 skipping SAM header line: @SQ SN:PGA_scaffold82846__1_contigs__length_30206 LN:30206 skipping SAM header line: @SQ SN:PGA_scaffold82847__1_contigs__length_30206 LN:30206 skipping SAM header line: @SQ SN:PGA_scaffold83718__1_contigs__length_30091 LN:30091 skipping SAM header line: @SQ SN:PGA_scaffold83719__1_contigs__length_30091 LN:30091 skipping SAM header line: @SQ SN:PGA_scaffold83732__1_contigs__length_30091 LN:30091 skipping SAM header line: @SQ SN:PGA_scaffold83733__1_contigs__length_30091 LN:30091 skipping SAM header line: @SQ SN:PGA_scaffold84570__1_contigs__length_34138 LN:34138 skipping SAM header line: @SQ SN:PGA_scaffold84571__1_contigs__length_34138 LN:34138 skipping SAM header line: @SQ SN:PGA_scaffold86623__1_contigs__length_31971 LN:31971 skipping SAM header line: @SQ SN:PGA_scaffold86624__1_contigs__length_31971 LN:31971 skipping SAM header line: @SQ 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SAM header line: @SQ SN:PGA_scaffold97179__1_contigs__length_37332 LN:37332 skipping SAM header line: @SQ SN:PGA_scaffold97181__1_contigs__length_41673 LN:41673 skipping SAM header line: @SQ SN:PGA_scaffold97182__1_contigs__length_37332 LN:37332 skipping SAM header line: @SQ SN:PGA_scaffold97184__1_contigs__length_41673 LN:41673 skipping SAM header line: @SQ SN:PGA_scaffold99123__1_contigs__length_38482 LN:38482 skipping SAM header line: @SQ SN:PGA_scaffold99127__1_contigs__length_38482 LN:38482 skipping SAM header line: @SQ SN:PGA_scaffold99731__1_contigs__length_75786 LN:75786 skipping SAM header line: @SQ SN:PGA_scaffold99732__1_contigs__length_75786 LN:75786 skipping SAM header line: @SQ SN:PGA_scaffold99735__1_contigs__length_75899 LN:75899 skipping SAM header line: @SQ SN:PGA_scaffold99736__1_contigs__length_75899 LN:75899 skipping SAM header line: @SQ SN:PGA_scaffold101565__1_contigs__length_30499 LN:30499 skipping SAM header line: @SQ 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LN:2213805 skipping SAM header line: @SQ SN:PGA_scaffold313646__4_contigs__length_6274882 LN:6274882 skipping SAM header line: @SQ SN:PGA_scaffold313647__2_contigs__length_5689983 LN:5689983 skipping SAM header line: @SQ SN:PGA_scaffold313648__2_contigs__length_3786466 LN:3786466 skipping SAM header line: @SQ SN:PGA_scaffold313649__2_contigs__length_2716785 LN:2716785 skipping SAM header line: @PG ID:Bismark VN:v0.19.0 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64 --samtools_path /gscratch/srlab/programs/samtools-1.9 --score_min L,0,-1.2 -I 60 -p 28 --genome /gscratch/srlab/strigg/data/Pgenr/Pgenr_070_30K/ -1 /gscratch/srlab/strigg/data/Pgenr/FASTQS/data_for_genome_comparison/EPI-120_S32_L005_R1_001_val_1.fq.gz -2 /gscratch/srlab/strigg/data/Pgenr/FASTQS/data_for_genome_comparison/EPI-120_S32_L005_R2_001_val_2.fq.gz -o /gscratch/srlab/strigg/analyses/20190214_30K" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed 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Processed lines: 11000000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 11000000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 10500000 Processed lines: 11000000 Processed lines: 11500000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Finished processing child process. Exiting.. Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11500000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11000000 Processed lines: 11500000 Processed lines: 11000000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Finished processing child process. Exiting.. Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Finished processing child process. Exiting.. Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Processed lines: 11500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 11500000 Finished processing child process. Exiting.. Processed lines: 11500000 Processed lines: 11500000 Finished processing child process. Exiting.. Processed lines: 11500000 Finished processing child process. Exiting.. Processed lines: 11500000 Finished processing child process. Exiting.. Processed lines: 11500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Now waiting for all child processes to complete Merging individual splitting reports into overall report: '/gscratch/srlab/strigg/analyses/20190214_30K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: Processed 11806794 lines in total Total number of methylation call strings processed: 23613588 Final Cytosine Methylation Report ================================= Total number of C's analysed: 275846395 Total methylated C's in CpG context: 9522649 Total methylated C's in CHG context: 1364555 Total methylated C's in CHH context: 3110238 Total C to T conversions in CpG context: 28107159 Total C to T conversions in CHG context: 55763455 Total C to T conversions in CHH context: 177978339 C methylated in CpG context: 25.3% C methylated in CHG context: 2.4% C methylated in CHH context: 1.7% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 101 Maximum read length of Read 2: 99 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 101 Maximum read length of Read 2: 99 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CpG_CTOB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHG_CTOB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHH_CTOB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt was empty -> deleted /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CpG_OB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHG_OB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt /gscratch/srlab/strigg/analyses/20190214_30K/CHH_OB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >/gscratch/srlab/strigg/analyses/20190214_30K/< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 2G Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: Writing bedGraph to file: EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Changed directory to /gscratch/srlab/strigg/analyses/20190214_30K/ The genome of interest was specified to contain gazillions of chromosomes or scaffolds. Merging all input files and sorting everything in memory instead of writing out individual chromosome files... Writing all merged methylation calls to temp file EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz.methylation_calls.merged Finished writing methylation calls from CpG_OT_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt to merged temp file Finished writing methylation calls from CpG_OB_EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt to merged temp file Sorting input file EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz.methylation_calls.merged by positions (using -S of 2G) Successfully deleted the temporary input file EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz.methylation_calls.merged Finished BedGraph conversion ...