Output will be written into the directory: /gscratch/srlab/strigg/analyses/20190214_10K/ Processing paired-end Bismark output file(s) (SAM format): /gscratch/srlab/strigg/analyses/20190214_10K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.bam /gscratch/srlab/strigg/analyses/20190214_10K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.bam /gscratch/srlab/strigg/analyses/20190214_10K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.bam /gscratch/srlab/strigg/analyses/20190214_10K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.bam If there are several alignments to a single position in the genome the first alignment will be chosen. Since the input files are not in any way sorted this is a near-enough random selection of reads. Checking file >>/gscratch/srlab/strigg/analyses/20190214_10K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/srlab/strigg/analyses/20190214_10K/EPI-111_S29_L005_R1_001_val_1_bismark_bt2_pe.bam: 13025293 Total number duplicated alignments removed: 2492731 (19.14%) Duplicated alignments were found at: 973800 different position(s) Total count of deduplicated leftover sequences: 10532562 (80.86% of total) Checking file >>/gscratch/srlab/strigg/analyses/20190214_10K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/srlab/strigg/analyses/20190214_10K/EPI-113_S30_L005_R1_001_val_1_bismark_bt2_pe.bam: 15835414 Total number duplicated alignments removed: 2965373 (18.73%) Duplicated alignments were found at: 1159592 different position(s) Total count of deduplicated leftover sequences: 12870041 (81.27% of total) Checking file >>/gscratch/srlab/strigg/analyses/20190214_10K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/srlab/strigg/analyses/20190214_10K/EPI-119_S31_L005_R1_001_val_1_bismark_bt2_pe.bam: 16928820 Total number duplicated alignments removed: 3232662 (19.10%) Duplicated alignments were found at: 1272082 different position(s) Total count of deduplicated leftover sequences: 13696158 (80.90% of total) Checking file >>/gscratch/srlab/strigg/analyses/20190214_10K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/srlab/strigg/analyses/20190214_10K/EPI-120_S32_L005_R1_001_val_1_bismark_bt2_pe.bam: 15041361 Total number duplicated alignments removed: 2670153 (17.75%) Duplicated alignments were found at: 1083772 different position(s) Total count of deduplicated leftover sequences: 12371208 (82.25% of total)