Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/data_for_genome_comparison/EPI-119_S31_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/data_for_genome_comparison/EPI-119_S31_L005_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Pgenr/Pgenr_070_10K/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 28905983 Number of paired-end alignments with a unique best hit: 16962818 Mapping efficiency: 58.7% Sequence pairs with no alignments under any condition: 7651911 Sequence pairs did not map uniquely: 4291254 Sequence pairs which were discarded because genomic sequence could not be extracted: 33998 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8601516 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 8327304 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 544456988 Total methylated C's in CpG context: 15192805 Total methylated C's in CHG context: 2451365 Total methylated C's in CHH context: 5312411 Total methylated C's in Unknown context: 165184 Total unmethylated C's in CpG context: 65321692 Total unmethylated C's in CHG context: 113561366 Total unmethylated C's in CHH context: 342617349 Total unmethylated C's in Unknown context: 2927480 C methylated in CpG context: 18.9% C methylated in CHG context: 2.1% C methylated in CHH context: 1.5% C methylated in unknown context (CN or CHN): 5.3% Bismark completed in 0d 6h 47m 56s