*** Bismark methylation extractor version v0.19.0 *** Setting option '--no_overlap' since this is (normally) the right thing to do for paired-end data Core usage currently set to more than 20 threads. Let's see how this goes... (set value: 28) Summarising Bismark methylation extractor parameters: =============================================================== Bismark paired-end SAM format specified (default) Number of cores to be used: 28 Output path specified as: /gscratch/srlab/strigg/analyses/20181101/ Summarising bedGraph parameters: =============================================================== Generating additional output in bedGraph and coverage format bedGraph format: coverage format: Using a cutoff of 1 read(s) to report cytosine positions Reporting and sorting cytosine methylation information in CpG context only (default) The bedGraph UNIX sort command will use the following memory setting: '2G'. Temporary directory used for sorting is the output directory Checking file >>/gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... ...passed! Writing result file containing methylation information for C in CpG context from the original top strand to /gscratch/srlab/strigg/analyses/20181101/CpG_OT_EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20181101/CpG_CTOT_EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20181101/CpG_CTOB_EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CpG context from the original bottom strand to /gscratch/srlab/strigg/analyses/20181101/CpG_OB_EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the original top strand to /gscratch/srlab/strigg/analyses/20181101/CHG_OT_EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20181101/CHG_CTOT_EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20181101/CHG_CTOB_EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHG context from the original bottom strand to /gscratch/srlab/strigg/analyses/20181101/CHG_OB_EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the original top strand to /gscratch/srlab/strigg/analyses/20181101/CHH_OT_EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the complementary to original top strand to /gscratch/srlab/strigg/analyses/20181101/CHH_CTOT_EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the complementary to original bottom strand to /gscratch/srlab/strigg/analyses/20181101/CHH_CTOB_EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in CHH context from the original bottom strand to /gscratch/srlab/strigg/analyses/20181101/CHH_OB_EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file /gscratch/srlab/strigg/analyses/20181101/EPI-103_S27_L005_R1_001_val_1_bismark_bt2_pe.deduplicated.bam skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:PGA_scaffold1__77_contigs__length_89643857 LN:89643857 skipping SAM header line: @SQ 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